Detailed information of TA system TA198006

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 45755..46482
• Replicon: plasmid pAW1.7_2
• Accession: NZ_CP072541
• Organism: Escherichia coli O128ac:H12 strain AW1.7

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A663AUB1
• Locus tag: J7886_RS24750
• Protein ID: WP_000558568.1
• Coordinates: 46171..46482 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: V0AHC4
• Locus tag: J7886_RS24745
• Protein ID: WP_000990392.1
• Coordinates: 45755..46174 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J7886_RS25330	41161..41491	-	331	Protein_38	type II toxin-antitoxin system RelE/ParE family toxin	-
J7886_RS24715 (J7886_24710)	41478..41784	-	307	Protein_39	type II toxin-antitoxin system ParD family antitoxin	-
J7886_RS24725 (J7886_24720)	42970..43779	-	810	WP_054623452.1	hypothetical protein	-
J7886_RS24730 (J7886_24725)	43797..44126	-	330	WP_109850802.1	hypothetical protein	-
J7886_RS24735 (J7886_24730)	44117..44769	+	653	Protein_43	IS6 family transposase	-
J7886_RS25335	44894..45718	+	825	WP_001141174.1	hypothetical protein	-
J7886_RS24745 (J7886_24740)	45755..46174	-	420	WP_000990392.1	helix-turn-helix domain-containing protein	Antitoxin
J7886_RS24750 (J7886_24745)	46171..46482	-	312	WP_000558568.1	type II toxin-antitoxin system HigB family toxin	Toxin
J7886_RS24755 (J7886_24750)	46712..49604	-	2893	Protein_47	Tn3 family transposase	-
J7886_RS24760 (J7886_24755)	49669..50373	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
J7886_RS24765 (J7886_24760)	50425..50661	-	237	Protein_49	transposase	-
J7886_RS24770 (J7886_24765)	50821..51084	+	264	WP_001295708.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..66909	66909
	-	inside	IScluster/Tn	-	-	32007..50373	18366

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12372.11 Da
• Isoelectric Point: 10.0935

>T198006 WP_000558568.1 NZ_CP072541:c46482-46171 [Escherichia coli O128ac:H12]
MHVVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Nucleotide

• Length: 312 bp

>T198006 NZ_CP096839:c2386945-2386628 [Mycobacterium tuberculosis variant bovis]
ATGACGCGCAGGCTGCGCGTCCATAACGGGGTTGAAGACGACCTATTCGAGGCGTTTTCCTACTACGCGGACGCGGCGCC
AGATCAGATCGATCGGCTTTACAACTTGTTTGTCGATGCCGTGACGAAGCGGATTCCGCAGGCTCCGAACGCGTTTGCGC
CGTTATTCAAGCACTATCGACACATCTACCTCCGGCCCTTCAGGTACTACGTTGCCTATCGGACGACCGACGAGGCTATC
GACATACTGGCTGTTCGGCACGGAATGGAGAACCCGAACGCGGTCGAGGCTGAGATCTCTGGCCGCACCTTCGAGTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15534.71 Da
• Isoelectric Point: 4.4702

>AT198006 WP_000990392.1 NZ_CP072541:c46174-45755 [Escherichia coli O128ac:H12]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Nucleotide

• Length: 420 bp

>AT198006 NZ_CP096839:c2387157-2386942 [Mycobacterium tuberculosis variant bovis]
GTGGTGGTCAACCGGGCATTGCTGGCGAGCGTCGACGCACTGTCGCGTGATGAGCAGATTGAGCTCGTCGAGCACATCAA
CGGAAACCTAGCCGAGGGCATGCATATCAGCGAGGCCAACCAGGCGCTCATCGAAGCGCGGGCCAATGACACCGACGATG
CTCATTGGTCCACCATTGATGACTTCGACAAGCGGATCCGCGCCCGGCTCGGATGA

Structures

Toxin

• AlphaFold DB: A0A663AUB1

Antitoxin

• AlphaFold DB: V0AHC4