Detailed information of TA system TA197984

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1218008..1218735
• Replicon: chromosome
• Accession: NZ_CP072539
• Organism: Escherichia coli O128ac:H12 strain AW1.7

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: J7886_RS05735
• Protein ID: WP_000547564.1
• Coordinates: 1218008..1218319 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J7886_RS05740
• Protein ID: WP_000126294.1
• Coordinates: 1218316..1218735 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J7886_RS05705 (1213150)	1213150..1214859	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
J7886_RS05710 (1214869)	1214869..1215411	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
J7886_RS05715 (1215411)	1215411..1216178	+	768	WP_000067401.1	formate hydrogenlyase subunit HycG	-
J7886_RS05720 (1216175)	1216175..1216585	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
J7886_RS05725 (1216578)	1216578..1217048	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
J7886_RS05730 (1217073)	1217073..1217834	+	762	WP_001026446.1	hypothetical protein	-
J7886_RS05735 (1218008)	1218008..1218319	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
J7886_RS05740 (1218316)	1218316..1218735	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
J7886_RS05745 (1218849)	1218849..1220273	-	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
J7886_RS05750 (1220282)	1220282..1221739	-	1458	WP_001107882.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
J7886_RS05755 (1221999)	1221999..1223009	+	1011	WP_001402444.1	DNA-binding transcriptional regulator AscG	-
J7886_RS05760 (1223158)	1223158..1223685	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T197984 WP_000547564.1 NZ_CP072539:1218008-1218319 [Escherichia coli O128ac:H12]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T197984 NZ_CP096839:839989-840417 [Mycobacterium tuberculosis variant bovis]
ATGTTCCTTCTCGACGCCAACGTGCTGCTGGCTGCACACCGCGGTGACCACCCGAATCACCGAACCGTCCGCCCCTGGTT
CGATCGACTGCTCGCGGCTGACGACCCCTTCACAGTGCCGAACCTGGTATGGGCGTCGTTCCTCCGGCTGGCAACGAATC
GACGCATCTTCGAGATTCCGTCACCGCGAGCAGAGGCATTCGCATTCGTCGAAGCCGTCACCGCCCAGCCCCATCACCTT
CCGACGAACCCCGGTCCCAGACACCTCATGCTGCTGCGAAAACTCTGCGACGAGGCCGACGCATCGGGCGACTTGATACC
TGACGCGGTACTCGCGGCCATAGCAGTGGGGCATCACTGCGCCGTGGTGAGCCTGGACAGGGATTTCGCCCGGTTTGCCT
CGGTGCGCCACATTCGCCCGCCGCTCTAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT197984 WP_000126294.1 NZ_CP072539:1218316-1218735 [Escherichia coli O128ac:H12]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT197984 NZ_CP096839:839708-839965 [Mycobacterium tuberculosis variant bovis]
ATGCGCACCACGGTGTCAATCTCCGATGAAATACTCGCTGCCGCCAAACGCCGGGCCCGCGAGCGTGGTCAATCGCTGGG
CGCTGTGATCGAGGACGCCCTTCGGCGGGAGTTCGCCGCCGCCCACGTCGGCGGCGCCCGCCCGACCGTCCCGGTTTTCG
ACGGCGGCACCGGTCCGCGGCGAGGCATCGACCTGACCTCGAATAGAGCGTTGTCCGAAGTGCTCGACGAGGGCCTGGAA
CTGAACTCCCGGAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5