Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 785121..785920 Replicon chromosome
Accession NZ_CP072539
Organism Escherichia coli O128ac:H12 strain AW1.7

Toxin (Protein)


Gene name yhaV Uniprot ID D7XZ20
Locus tag J7886_RS03640 Protein ID WP_000347264.1
Coordinates 785121..785585 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag J7886_RS03645 Protein ID WP_001307405.1
Coordinates 785585..785920 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J7886_RS03610 (780122) 780122..780556 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
J7886_RS03615 (780574) 780574..781452 - 879 WP_001300474.1 PTS N-acetylgalactosamine transporter subunit IID -
J7886_RS03620 (781442) 781442..782221 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
J7886_RS03625 (782232) 782232..782705 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
J7886_RS03630 (782728) 782728..784008 - 1281 WP_054625916.1 tagatose-bisphosphate aldolase subunit KbaZ -
J7886_RS03635 (784257) 784257..785066 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
J7886_RS03640 (785121) 785121..785585 - 465 WP_000347264.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
J7886_RS03645 (785585) 785585..785920 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
J7886_RS03650 (786069) 786069..787640 - 1572 WP_001273753.1 galactarate dehydratase -
J7886_RS03655 (788015) 788015..789349 + 1335 WP_054625915.1 galactarate/glucarate/glycerate transporter GarP -
J7886_RS03660 (789365) 789365..790135 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17894.29 Da        Isoelectric Point: 9.4945

>T197979 WP_000347264.1 NZ_CP072539:c785585-785121 [Escherichia coli O128ac:H12]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFDAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T197979 NZ_CP096839:716193-716600 [Mycobacterium tuberculosis variant bovis]
GTGAGCACGACGCCGGCCGCCGGAGTGCTCGACACGTCGGTGTTCATCGCGACCGAAAGCGGCCGGCAACTCGACGAGGC
GCTGATCCCCGACCGGGTCGCCACCACCGTCGTCACCCTCGCCGAACTGCGCGTCGGCGTGCTGGCCGCGGCGACGACCG
ACATCCGGGCTCAACGCCTGGCGACCCTGGAATCCGTTGCCGATATGGAAACGTTGCCCGTCGACGACGATGCCGCCCGA
ATGTGGGCCCGATTGCGGATCCATCTTGCCGAGTCCGGTCGCCGGGTGCGGATCAACGACCTGTGGATCGCGGCCGTCGC
GGCATCGCGAGCGCTGCCGGTCATCACCCAGGACGACGACTTCGCCGCCCTCGACGGTGCGGCCAGTGTGGAGATCATTC
GGGTCTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT197979 WP_001307405.1 NZ_CP072539:c785920-785585 [Escherichia coli O128ac:H12]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT197979 NZ_CP096839:715936-716196 [Mycobacterium tuberculosis variant bovis]
ATGTCTGAGGTGGCCTCGCGTGAGCTGCGTAACGATACGGCCGGCGTGCTGCGCCGCGTGCGGGCAGGGGAGGACGTCAC
CATCACCGTCAGCGGCCGTCCGGTCGCGGTGCTTACCCCGGTTCGTCCGCGGCGCCGGCGTTGGCTGAGCAAAACGGAGT
TCCTGTCGCGGTTGCGCGGCGCTCAAGCCGATCCCGGGCTCCGTAACGACCTCGCGGTCCTTGCCGGCGACACGACCGAG
GATCTCGGGCCGATCCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A241QML2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References