Detailed information of TA system TA197975

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 69120..69705
• Replicon: plasmid pDETAB5
• Accession: NZ_CP072528
• Organism: Acinetobacter baumannii strain DETAB-E227

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9CJF7
• Locus tag: J7D37_RS18800
• Protein ID: WP_000897307.1
• Coordinates: 69120..69440 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J7D37_RS18805
• Protein ID: WP_000369781.1
• Coordinates: 69433..69705 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J7D37_RS18785 (J7D37_18785)	65419..66933	+	1515	WP_032073148.1	MFS transporter	-
J7D37_RS18790 (J7D37_18790)	67037..68143	-	1107	WP_032073131.1	two-component sensor histidine kinase AdeS	-
J7D37_RS18795 (J7D37_18795)	68133..68861	-	729	WP_032073130.1	efflux system response regulator transcription factor AdeR	-
J7D37_RS18800 (J7D37_18800)	69120..69440	+	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J7D37_RS18805 (J7D37_18805)	69433..69705	+	273	WP_000369781.1	NadS family protein	Antitoxin
J7D37_RS18810 (J7D37_18810)	70198..71673	+	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
J7D37_RS18815 (J7D37_18815)	71729..72613	+	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
J7D37_RS18820 (J7D37_18820)	72804..73019	-	216	Protein_71	helix-turn-helix domain-containing protein	-
J7D37_RS18825 (J7D37_18825)	73072..73776	+	705	WP_005028420.1	IS6 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(3)-IId / blaOXA-58 / sul2 / msr(E) / mph(E) / blaNDM-1	-	1..97035	97035
	-	flank	IS/Tn	msr(E) / mph(E)	-	70198..73776	3578

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12187.95 Da
• Isoelectric Point: 7.1639

>T197975 WP_000897307.1 NZ_CP072528:69120-69440 [Acinetobacter baumannii]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILHQLVKEQFHG

Nucleotide

• Length: 321 bp

>T197975 NZ_CP096839:c695477-695064 [Mycobacterium tuberculosis variant bovis]
GTGAAACCGCCGCTGGCAGTGGACACCAGCGTCGCGATTCCGTTGCTGGTACGGACCCATACCGCGCATGCGGCGGTTGT
CGCCTGGTGGGCACATCGGGAGGCCGCGCTGTGCGGGCACGCCCTGGCCGAAACCTACTCCGTGCTGACCCGCCTGCCGC
GTGACCTACGACTGGCACCGATGGACGCCGCGCGGCTTCTTACCGAACGCTTTGCGGCACCGCTGTTGTTGAGTTCCCGC
ACCACTGAACATCTCCCACGCGTGCTGGCCCAATTCGAGATCACTGGTGGCGCGGTCTATGACGCTCTCGTGGCCCTTGC
CGCCGCCGAGCACCGGGCTGAACTCGCCACACGCGATGCTCGAGCCAAGGACACTTACGAAAAGATCGGCGTCCACGTCG
TTGTCGCCGCGTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT197975 WP_000369781.1 NZ_CP072528:69433-69705 [Acinetobacter baumannii]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT197975 NZ_CP096839:c695710-695474 [Mycobacterium tuberculosis variant bovis]
ATGAAGGCTGTTGTGGATGCGGCTGGCCGCATCGTCGTACCCAAACCGCTGCGCGAAGCCTTGGGGCTGCAACCGGGGTC
CACCGTGGAAATATCCCGCTATGGCGCTGGTCTCCACCTGATTCCCACCGGACGAACAGCGCGTCTCGAAGAGGAGAATG
GCGTACTGGTGGCCACCGGTGAGACCACGATCGACGATGAGGTCGTGTTCGGCCTTATCGATAGCGGCCGGAAGTGA

Structures

Toxin

• AlphaFold DB: A0A0A8TMZ3