Detailed information of TA system TA197959

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 163128..163785
• Replicon: chromosome
• Accession: NZ_CP072521
• Organism: Burkholderia seminalis strain 869T2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A8A8DAZ5
• Locus tag: DT99_RS17325
• Protein ID: WP_034189650.1
• Coordinates: 163128..163460 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DT99_RS17330
• Protein ID: WP_034189649.1
• Coordinates: 163453..163785 (+)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DT99_RS17310 (DT99_017310)	158850..159041	+	192	WP_154233779.1	hypothetical protein	-
DT99_RS17315 (DT99_017315)	159163..162501	+	3339	WP_245169498.1	autotransporter outer membrane beta-barrel domain-containing protein	-
DT99_RS17320 (DT99_017320)	162641..162790	+	150	WP_154233778.1	hypothetical protein	-
DT99_RS17325 (DT99_017325)	163128..163460	+	333	WP_034189650.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DT99_RS17330 (DT99_017330)	163453..163785	+	333	WP_034189649.1	helix-turn-helix transcriptional regulator	Antitoxin
DT99_RS17335 (DT99_017335)	163841..164680	-	840	WP_034189648.1	AraC family transcriptional regulator	-
DT99_RS17340 (DT99_017340)	164829..165563	-	735	WP_034189647.1	fucose-binding lectin II	-
DT99_RS17345 (DT99_017345)	165714..166520	-	807	WP_034189646.1	fucose-binding lectin II	-
DT99_RS17350 (DT99_017350)	166644..167033	-	390	WP_034189645.1	fucose-binding lectin II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (18-104)

Antitoxin

• Cro/C1-type helix-turn-helix domain (52-105)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12901.03 Da
• Isoelectric Point: 10.0669

>T197959 WP_034189650.1 NZ_CP072521:163128-163460 [Burkholderia seminalis]
MKKWTILYYDERVKRDVFALPKGILAGYLRLIETMEEYGADLRMPHSRAMGDGLFELRPRGREGIGRVFYCMQVGYDLVV
LHSFVKKTQETSGDELRIARRRMKEVRNNG

Nucleotide

• Length: 333 bp

>T197959 NZ_CP096837:c2437252-2437076 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGATGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 11951.49 Da
• Isoelectric Point: 8.0754

>AT197959 WP_034189649.1 NZ_CP072521:163453-163785 [Burkholderia seminalis]
MAKARHAHASEERYAPVRHTAEDTARLLADPAIKAEYDALEEEFTALRALLDARKDAGLTQAQVAERMGTTTSAVSRLEA
SFSSEKHSPSFATLRKYAAACGKKLVISFA

Nucleotide

• Length: 333 bp

>AT197959 NZ_CP096837:c2437030-2436614 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A8A8DAZ5