Detailed information of TA system TA197942

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 1233480..1234077
• Replicon: chromosome
• Accession: NZ_CP072515
• Organism: Klebsiella quasipneumoniae strain 3Z-5L

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: J6325_RS05740
• Protein ID: WP_108450630.1
• Coordinates: 1233480..1233797 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J6325_RS05745
• Protein ID: WP_108450629.1
• Coordinates: 1233790..1234077 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6325_RS05725 (1229314)	1229314..1230741	-	1428	WP_109938140.1	MFS transporter	-
J6325_RS05730 (1230850)	1230850..1231719	+	870	WP_017900842.1	helix-turn-helix transcriptional regulator	-
J6325_RS05735 (1232252)	1232252..1233295	+	1044	WP_060656299.1	hypothetical protein	-
J6325_RS05740 (1233480)	1233480..1233797	+	318	WP_108450630.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J6325_RS05745 (1233790)	1233790..1234077	+	288	WP_108450629.1	helix-turn-helix transcriptional regulator	Antitoxin
J6325_RS05750 (1234335)	1234335..1234988	-	654	WP_108450628.1	oxygen-insensitive NAD(P)H nitroreductase	-
J6325_RS05755 (1235108)	1235108..1235476	-	369	WP_004202487.1	MmcQ/YjbR family DNA-binding protein	-
J6325_RS05760 (1235476)	1235476..1236057	-	582	WP_017899014.1	TetR/AcrR family transcriptional regulator	-
J6325_RS05765 (1236237)	1236237..1237352	+	1116	WP_025999133.1	MBL fold metallo-hydrolase	-
J6325_RS05770 (1237383)	1237383..1237724	+	342	WP_004202492.1	RamA family antibiotic efflux transcriptional regulator	-
J6325_RS05775 (1237742)	1237742..1237990	-	249	WP_002893037.1	DUF1158 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12197.57 Da
• Isoelectric Point: 11.2767

>T197942 WP_108450630.1 NZ_CP072515:1233480-1233797 [Klebsiella quasipneumoniae]
MFRMVVHVDVKKELQALPAIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQNKTLFLLRVF
VKKTQKIPLSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T197942 NZ_CP096834:4089307-4089705 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTAACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10840.56 Da
• Isoelectric Point: 10.4518

>AT197942 WP_108450629.1 NZ_CP072515:1233790-1234077 [Klebsiella quasipneumoniae]
MSKKIIDWDTLKTELLSQPEVQAAFDAQERKHRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRTSLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT197942 NZ_CP096834:4089080-4089307 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA