Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 1135297..1135894 Replicon chromosome
Accession NZ_CP072494
Organism Klebsiella pneumoniae strain KPN246

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag J7V84_RS05535 Protein ID WP_004142563.1
Coordinates 1135297..1135614 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag J7V84_RS05540 Protein ID WP_004142561.1
Coordinates 1135607..1135894 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J7V84_RS26890 (1130581) 1130581..1131018 + 438 WP_004202377.1 hypothetical protein -
J7V84_RS05505 (1131246) 1131246..1131425 - 180 WP_225322758.1 AlpA family transcriptional regulator -
J7V84_RS26895 (1131849) 1131849..1132014 - 166 Protein_1085 hypothetical protein -
J7V84_RS05510 (1132292) 1132292..1132585 + 294 WP_002892360.1 hypothetical protein -
J7V84_RS05515 (1132981) 1132981..1133382 + 402 WP_002892366.1 hydroxyisourate hydrolase -
J7V84_RS05520 (1133461) 1133461..1133631 - 171 WP_002892370.1 hypothetical protein -
J7V84_RS05525 (1133956) 1133956..1134096 + 141 WP_004142684.1 hypothetical protein -
J7V84_RS05530 (1134174) 1134174..1135142 + 969 WP_004225014.1 IS5 family transposase -
J7V84_RS05535 (1135297) 1135297..1135614 + 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
J7V84_RS05540 (1135607) 1135607..1135894 + 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
J7V84_RS05545 (1136159) 1136159..1136812 - 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
J7V84_RS05550 (1136932) 1136932..1137300 - 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
J7V84_RS05555 (1137300) 1137300..1137881 - 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
J7V84_RS05560 (1138060) 1138060..1139175 + 1116 WP_172676897.1 MBL fold metallo-hydrolase -
J7V84_RS05565 (1139206) 1139206..1139547 + 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
J7V84_RS05570 (1139565) 1139565..1139813 - 249 WP_002893037.1 DUF1158 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1111783..1135894 24111
- flank IS/Tn - - 1134219..1135142 923


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T197901 WP_004142563.1 NZ_CP072494:1135297-1135614 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T197901 NZ_CP096821:1742653-1742973 [Pectobacterium sp. 21LCBS03]
ATGCAAACAACACCTGCAATCCCGCCACGGGAGGATAACCCCTGTCCGTCACCTATAACCGTCTGGCAACAACTGCTGAC
CTACCTGCTGGAAAAGCACTACGGCCTCACGCTGAATGACACACCGTTCGGCGAGGAGAACGTGATTCAGGCGCATATCG
ATGCTGGCGTCACGCTGGTCAATGCCGTCAATTTTCTGGTGGAAAAATACGAACTGGTGCGTATCGATCGCAACGGCTTT
AACTGGCAGGAGCAATCGCCGTTTCTTACCGCCGTGGATGTTCTCCGTGCCAGACGTGCTACCGGCTTGCTCAAGGCATA
A

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT197901 WP_004142561.1 NZ_CP072494:1135607-1135894 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT197901 NZ_CP096821:1742248-1742583 [Pectobacterium sp. 21LCBS03]
ATGTCATCAATAGAGGCCTCGGAATGGGGATTAAAGTGTGCTGTCACGCCGCGATTCGGAGCCAGACTGGTGCAGGAAGG
TAACCGTCTGCACTATCTGGCAGACCGGGCCAGCTTTATCGGTACGTTTAGCAAGGCAGAAGCCCGTAATCTGGAACACG
TCTTCCCTGAGCAGATTAAGCAGTTGGAGCGGAAACTACGCACGGGTGAACTGAATCCTCGTCAGCAGAACTGCGTCACG
CTGCATTGCAACGAATGGATCTGTGAAGCCGATACGCTGGGCAGTGTTGGCTACGTGTATATTGCTATTTATCCCTCTTC
AGCGGCAACACAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References