Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 324027..324613 Replicon chromosome
Accession NZ_CP072494
Organism Klebsiella pneumoniae strain KPN246

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag J7V84_RS01510 Protein ID WP_002920800.1
Coordinates 324245..324613 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag J7V84_RS01505 Protein ID WP_004174006.1
Coordinates 324027..324248 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J7V84_RS01485 (320184) 320184..321110 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
J7V84_RS01490 (321107) 321107..322384 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
J7V84_RS01495 (322381) 322381..323148 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
J7V84_RS01500 (323150) 323150..323863 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
J7V84_RS01505 (324027) 324027..324248 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
J7V84_RS01510 (324245) 324245..324613 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
J7V84_RS01515 (324886) 324886..326202 + 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
J7V84_RS01520 (326309) 326309..327196 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
J7V84_RS01525 (327193) 327193..328038 + 846 WP_088498490.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
J7V84_RS01530 (328040) 328040..329110 + 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 319013..329847 10834


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T197899 WP_002920800.1 NZ_CP072494:324245-324613 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T197899 NZ_CP096819:5665-5952 [Acinetobacter baumannii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCAATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATCCGAATCATTAGT
GCAAGGCAAGTCACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT197899 WP_004174006.1 NZ_CP072494:324027-324248 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT197899 NZ_CP096819:5939-6253 [Acinetobacter baumannii]
ATGAGCATGGTTAGATACTCACACAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAGCAATGCTGTACGCC
AAAATCAATTTTACCGCCCAGTAAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGTATGAATGAAATTTTACGAGATGCTATGCTAAAAGAATTAAAAAATCATCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References