Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/HigB-HigA |
| Location | 57423..58150 | Replicon | plasmid unnamed1 |
| Accession | NZ_CP072493 | ||
| Organism | Klebsiella pneumoniae strain KPN236 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | A0A7W3D9W1 |
| Locus tag | J7V87_RS26045 | Protein ID | WP_011251285.1 |
| Coordinates | 57839..58150 (-) | Length | 104 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | J7V87_RS26040 | Protein ID | WP_011251286.1 |
| Coordinates | 57423..57842 (-) | Length | 140 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| J7V87_RS26010 (J7V87_26005) | 52591..53061 | - | 471 | WP_048333570.1 | hypothetical protein | - |
| J7V87_RS26015 (J7V87_26010) | 53374..54009 | - | 636 | WP_223171879.1 | hypothetical protein | - |
| J7V87_RS26020 (J7V87_26015) | 54428..55048 | + | 621 | WP_223175004.1 | conjugative transfer protein MobI(A/C) | - |
| J7V87_RS26025 (J7V87_26020) | 55069..55857 | + | 789 | WP_040217257.1 | hypothetical protein | - |
| J7V87_RS26030 (J7V87_26025) | 55871..56236 | + | 366 | WP_048333448.1 | hypothetical protein | - |
| J7V87_RS26035 (J7V87_26030) | 56308..57276 | - | 969 | WP_011251287.1 | IS5 family transposase | - |
| J7V87_RS26040 (J7V87_26035) | 57423..57842 | - | 420 | WP_011251286.1 | helix-turn-helix domain-containing protein | Antitoxin |
| J7V87_RS26045 (J7V87_26040) | 57839..58150 | - | 312 | WP_011251285.1 | type II toxin-antitoxin system HigB family toxin | Toxin |
| J7V87_RS26050 (J7V87_26045) | 58355..58792 | - | 438 | Protein_58 | DDE-type integrase/transposase/recombinase | - |
| J7V87_RS26055 (J7V87_26050) | 58927..59624 | + | 698 | WP_223175001.1 | IS1-like element IS1A family transposase | - |
| J7V87_RS26060 (J7V87_26055) | 59626..60075 | + | 450 | WP_011251283.1 | thioredoxin fold domain-containing protein | - |
| J7V87_RS26065 (J7V87_26060) | 60092..60403 | + | 312 | WP_011251282.1 | hypothetical protein | - |
| J7V87_RS26070 (J7V87_26065) | 60417..60758 | + | 342 | WP_011251281.1 | hypothetical protein | - |
| J7V87_RS26075 (J7V87_26070) | 60818..61246 | + | 429 | WP_228293358.1 | hypothetical protein | - |
| J7V87_RS26080 (J7V87_26075) | 61283..62251 | - | 969 | WP_004225014.1 | IS5 family transposase | - |
| J7V87_RS26085 (J7V87_26080) | 62231..62839 | + | 609 | WP_228293359.1 | DsbA family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | - | iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2 | 1..231163 | 231163 | |
| - | inside | IScluster/Tn | - | - | 47302..62206 | 14904 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 104 a.a. Molecular weight: 12346.12 Da Isoelectric Point: 9.7248
>T197896 WP_011251285.1 NZ_CP072493:c58150-57839 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE
Download Length: 312 bp
>T197896 NZ_CP096817:c38103-37687 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAATAA
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAATAA
Antitoxin
Download Length: 140 a.a. Molecular weight: 15377.46 Da Isoelectric Point: 4.4420
>AT197896 WP_011251286.1 NZ_CP072493:c57842-57423 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL
Download Length: 420 bp
>AT197896 NZ_CP096817:c38330-38100 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|