Detailed information of TA system TA197857

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 96540..96809
• Replicon: plasmid unnamed2
• Accession: NZ_CP072465
• Organism: Klebsiella pneumoniae strain KP1517

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: HRG83_RS29045
• Protein ID: WP_001372321.1
• Coordinates: 96684..96809 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	96540..96605 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRG83_RS29020	92250..92777	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
HRG83_RS29025	92835..93068	+	234	WP_000006003.1	DUF905 family protein	-
HRG83_RS29030	93129..95152	+	2024	Protein_129	ParB/RepB/Spo0J family partition protein	-
HRG83_RS29035	95221..95655	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
HRG83_RS29040	95652..96371	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	96383..96607	+	225	NuclAT_0	-	-
-	96383..96607	+	225	NuclAT_0	-	-
-	96383..96607	+	225	NuclAT_0	-	-
-	96383..96607	+	225	NuclAT_0	-	-
-	96540..96605	-	66	-	-	Antitoxin
HRG83_RS29520	96593..96742	+	150	Protein_132	plasmid maintenance protein Mok	-
HRG83_RS29045	96684..96809	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
HRG83_RS29050	97128..97424	-	297	Protein_134	hypothetical protein	-
HRG83_RS29055	97724..98020	+	297	WP_001272251.1	hypothetical protein	-
HRG83_RS29060	98131..98952	+	822	WP_001234445.1	DUF932 domain-containing protein	-
HRG83_RS29065	99249..99896	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
HRG83_RS29070	100173..100556	+	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
HRG83_RS29075	100747..101433	+	687	WP_015059009.1	PAS domain-containing protein	-
HRG83_RS29080	101527..101754	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	fosA3 / rmtB / blaSHV-12 / blaKPC-2	-	1..119109	119109

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T197857 WP_001372321.1 NZ_CP072465:96684-96809 [Klebsiella pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T197857 NZ_CP096810:c2613149-2612199 [Klebsiella pneumoniae]
ATGGATGATTTGACAAAGCTATTCCCGGACGTTGCTGATGCTCTGGGCATTGAATCAGTTGCAATTGTAGAGAAAGATCA
TTACATCGTTGAGTTATTGCGATTGCTCCAGCCTCTGTCCTTTGATACACATCAGCTGGTTTTTGCCGGTGGAACAGCAT
TGTCTAAAGCGGGTATATCTTTGAACAGGATGTCGGAGGACGTGGACATTAAGCTTGTTCCTGTCACCGGCTTTTCCCTG
CAATATTCCAGAAATCAACGTAAAAATATCCGTAAAGATATTATTAAGACCATCACCGAAACGATTGTAGCTTCAGGTAT
GTTTAGCATGGATGAGGAGTACCCAAAGGTTACTCGTGATGAAAACCGCTATAATGAAATTCCGGTGCGCTACCCGCAGG
AATATGTACAAATTCCCTGTTTAAGGCCATTTATCAAACTGGAACTGATGGAAACGGATTTGCTTGAGGCTGCGGAAAAT
CGTGCTATTAGCTCACTGGTAATGGACCTAACAAAGAAGGGCGAAGTAGTGAGGGCTTTTCCCTGCGCAACTATCGGCAG
CACGCAGGCTGAAAAACTGATCTCGATGATGCGCAGAACGGCTGCAAGTATGCGTGATGTGGAGCGTGCAGTGGATGAGT
CACTGGTGCGTCATATCTACGACAACTTTTGTATTGTCAGAGAAAAAGGTGCTGAGGTTCCTGTTTTGAAGCGCTTTGTG
CAGGAATGTATTGAGCAGGATATCCAACGTTACGGTGCTCAGTATCCTGAATTCTGCGAGTCGCCTGTTGAAGAACTAAA
AATGGGGTTGGAAGAACTGGCGAGCAACCCTGTTTATAAGGAACGCTATCTGCAGTTTGTTACACCAATGGTGTTCGGTG
AAAGCCACGTTTCCTGGGAAGAGGCCTATGCCTGTTTTCGCATAACGGCATTGGATGTGATTGATGCATAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT197857 NZ_CP072465:c96605-96540 [Klebsiella pneumoniae]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin