Detailed information of TA system TA197854

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 67673..67926
• Replicon: plasmid unnamed2
• Accession: NZ_CP072465
• Organism: Klebsiella pneumoniae strain KP1517

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: HRG83_RS28855
• Protein ID: WP_001312851.1
• Coordinates: 67777..67926 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	67673..67732 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRG83_RS28815 (63333)	63333..63398	-	66	Protein_85	helix-turn-helix domain-containing protein	-
HRG83_RS28820 (63451)	63451..64155	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
HRG83_RS28825 (64180)	64180..64380	+	201	WP_072354025.1	hypothetical protein	-
HRG83_RS28830 (64400)	64400..65146	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
HRG83_RS28835 (65201)	65201..65761	+	561	WP_000139328.1	fertility inhibition protein FinO	-
HRG83_RS28840 (65893)	65893..66093	+	201	WP_015059022.1	hypothetical protein	-
HRG83_RS28845 (66479)	66479..67078	+	600	WP_032083981.1	PIN domain-containing protein	-
HRG83_RS28850 (67140)	67140..67472	+	333	WP_152916585.1	hypothetical protein	-
- (67673)	67673..67732	-	60	NuclAT_1	-	Antitoxin
- (67673)	67673..67732	-	60	NuclAT_1	-	Antitoxin
- (67673)	67673..67732	-	60	NuclAT_1	-	Antitoxin
- (67673)	67673..67732	-	60	NuclAT_1	-	Antitoxin
HRG83_RS28855 (67777)	67777..67926	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
HRG83_RS28860 (68210)	68210..68458	+	249	WP_000083837.1	replication regulatory protein RepA	-
HRG83_RS29500 (68573)	68573..68751	+	179	Protein_95	protein CopA/IncA	-
HRG83_RS28870 (68770)	68770..69627	+	858	WP_142295464.1	incFII family plasmid replication initiator RepA	-
HRG83_RS28875 (70566)	70566..71219	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
HRG83_RS28880 (71312)	71312..71569	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
HRG83_RS28885 (71502)	71502..71903	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
HRG83_RS28890 (72152)	72152..72567	+	416	Protein_100	IS1-like element IS1B family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	fosA3 / rmtB / blaSHV-12 / blaKPC-2	-	1..119109	119109

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T197854 WP_001312851.1 NZ_CP072465:67777-67926 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T197854 NZ_CP096810:c1915210-1914725 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGACCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Nucleotide

• Length: 60 bp

>AT197854 NZ_CP072465:c67732-67673 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin