Detailed information of TA system TA197820

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 2658944..2659541
• Replicon: chromosome
• Accession: NZ_CP072460
• Organism: Klebsiella pneumoniae strain K-1L

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: J7V83_RS13160
• Protein ID: WP_004893639.1
• Coordinates: 2659224..2659541 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: J7V83_RS13155
• Protein ID: WP_004142561.1
• Coordinates: 2658944..2659231 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J7V83_RS13125 (2655151)	2655151..2655399	+	249	WP_002893037.1	DUF1158 domain-containing protein	-
J7V83_RS13130 (2655417)	2655417..2655758	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
J7V83_RS13135 (2655789)	2655789..2656904	-	1116	WP_020804106.1	MBL fold metallo-hydrolase	-
J7V83_RS13140 (2657084)	2657084..2657665	+	582	WP_101853489.1	TetR/AcrR family transcriptional regulator	-
J7V83_RS13145 (2657665)	2657665..2658033	+	369	WP_004142557.1	MmcQ/YjbR family DNA-binding protein	-
J7V83_RS13150 (2658153)	2658153..2658806	+	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
J7V83_RS13155 (2658944)	2658944..2659231	-	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
J7V83_RS13160 (2659224)	2659224..2659541	-	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J7V83_RS13165 (2659726)	2659726..2660769	-	1044	WP_186593469.1	DUF2157 domain-containing protein	-
J7V83_RS13170 (2661439)	2661439..2662305	-	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
J7V83_RS13175 (2662414)	2662414..2663841	+	1428	WP_004176980.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2658944..2668098	9154

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T197820 WP_004893639.1 NZ_CP072460:c2659541-2659224 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T197820 NZ_CP096750:c7609-7253 [Acinetobacter baumannii]
ATGTGGACAGTCATTACAACGGATCTGTTTAATGAATGGCTTGAACAGCAAGATGAAGCAACACAAGAGAAAGTCTTAGC
TGCATTGGTTGTTCTACAACAGCAAGGGCCTAGTTTAGGTCGTCCTTTAGTAGATACCGTCTACGATTCTAAATTTACCA
ATATGAAGGAACTACGTGTTCAGCATCGTGGTAAACCGTTAAGGGCATTTTTTGCATTCGATCCCCTAAGACAAGCGATT
GTGCTGTGTATTGGTGATAAGAGTAATAAAAAGCGTTTTTATACAGAGATGCTAGCAATTGCAGATGAGCAATACGCACT
TCATCTCTCAACTTTAGGAGATCAATCTAATGGCTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT197820 WP_004142561.1 NZ_CP072460:c2659231-2658944 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT197820 NZ_CP096750:c7260-6958 [Acinetobacter baumannii]
ATGGCTAAGAGCTTACAAGAATTATTGGCTAGTCGCTCTCCAGAGAGTCAAGCTCGTATTCAAAAAATGGCTGATGAATT
ACTTTTAGAAAGTCAGCTTCACCTTATCCGAGAAGAGTTAGAAATTTCCCAGAAAGAACTAGCTGCAACCCTTGGAATTA
AACAGCCGTCATTATCAGCAATCGAAAATCGTGGTCATGATCTTAAAATTTCAACGATGAAAAAGTATGTTGAAGCAATG
GGTGGAAAACTTCGTATTGATGTAGAGCTTCCAACAGGAAAACACATAGGGTTCAATGTCTAA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1