Detailed information of TA system TA197803

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3920575..3921172
• Replicon: chromosome
• Accession: NZ_CP072456
• Organism: Klebsiella pneumoniae strain b1-2L

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: J7V71_RS19380
• Protein ID: WP_004893639.1
• Coordinates: 3920855..3921172 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: J7V71_RS19375
• Protein ID: WP_004142561.1
• Coordinates: 3920575..3920862 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J7V71_RS19345 (3916782)	3916782..3917030	+	249	WP_002893037.1	DUF1158 domain-containing protein	-
J7V71_RS19350 (3917048)	3917048..3917389	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
J7V71_RS19355 (3917420)	3917420..3918535	-	1116	WP_020804106.1	MBL fold metallo-hydrolase	-
J7V71_RS19360 (3918715)	3918715..3919296	+	582	WP_101853489.1	TetR/AcrR family transcriptional regulator	-
J7V71_RS19365 (3919296)	3919296..3919664	+	369	WP_004142557.1	MmcQ/YjbR family DNA-binding protein	-
J7V71_RS19370 (3919784)	3919784..3920437	+	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
J7V71_RS19375 (3920575)	3920575..3920862	-	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
J7V71_RS19380 (3920855)	3920855..3921172	-	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J7V71_RS19385 (3921357)	3921357..3922400	-	1044	WP_186593469.1	DUF2157 domain-containing protein	-
J7V71_RS19390 (3923070)	3923070..3923936	-	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
J7V71_RS19395 (3924045)	3924045..3925472	+	1428	WP_004176980.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T197803 WP_004893639.1 NZ_CP072456:c3921172-3920855 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T197803 NZ_CP096731:450848-451237 [Acinetobacter baumannii]
TTGATCAACCATTTGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AGTTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCACAAATTGCTTATTTAGATAAAAAACTATGGTCGCTTGCTTATAAGTCACAGAACACCGTT
TCTCGCGTCAAAATTTTAAAAATAATTGATTGTCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGCAGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCTATTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT197803 WP_004142561.1 NZ_CP072456:c3920862-3920575 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT197803 NZ_CP096731:450584-450841 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ACCCTGATTTACTAGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1