Detailed information of TA system TA197773

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1458250..1458974
• Replicon: chromosome
• Accession: NZ_CP072453
• Organism: Providencia sp. R33

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: J6836_RS06825
• Protein ID: WP_219247915.1
• Coordinates: 1458250..1458561 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J6836_RS06830
• Protein ID: WP_219247917.1
• Coordinates: 1458558..1458974 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6836_RS06810 (J6836_06815)	1454122..1455330	-	1209	WP_255586271.1	cupin domain-containing protein	-
J6836_RS06815 (J6836_06820)	1455278..1457575	-	2298	WP_219245543.1	bifunctional salicylyl-CoA 5-hydroxylase/oxidoreductase	-
J6836_RS06820 (J6836_06825)	1457592..1458011	-	420	WP_219247913.1	thioesterase family protein	-
J6836_RS06825 (J6836_06830)	1458250..1458561	+	312	WP_219247915.1	type II toxin-antitoxin system HigB family toxin	Toxin
J6836_RS06830 (J6836_06835)	1458558..1458974	+	417	WP_219247917.1	helix-turn-helix domain-containing protein	Antitoxin
J6836_RS06835 (J6836_06840)	1459020..1459958	-	939	WP_219247919.1	zinc-binding alcohol dehydrogenase family protein	-
J6836_RS06840 (J6836_06845)	1460028..1460492	+	465	WP_219247921.1	MarR family winged helix-turn-helix transcriptional regulator	-
J6836_RS06845 (J6836_06850)	1460469..1461053	-	585	WP_282560613.1	hypothetical protein	-
J6836_RS06850 (J6836_06855)	1461126..1461527	-	402	WP_255586341.1	RidA family protein	-
J6836_RS06855 (J6836_06860)	1461655..1461984	+	330	WP_282560671.1	helix-turn-helix transcriptional regulator	-
J6836_RS06860 (J6836_06865)	1461981..1463288	+	1308	WP_219247925.1	type II toxin-antitoxin system HipA family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12377.25 Da
• Isoelectric Point: 9.9151

>T197773 WP_219247915.1 NZ_CP072453:1458250-1458561 [Providencia sp. R33]
MHVISREPFELASVRFPKNAQAIIDAYRTLKRDAYRSPDELKQAFPSLDRMKYREKCWVIDIGGNTLRILFFADFEREKI
FVKHISTHAEYNKLVDHYRRTKE

Nucleotide

• Length: 312 bp

>T197773 NZ_CP096698:448646-449035 [Acinetobacter baumannii]
TTGATCAACCATTTGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AGTTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCACAAATTGCTTATTTAGATAAAAAACTATGGTCGCTTGCTTATAAGTCACAGAACACCGTT
TCTCGCGTCAAAATTTTAAAAATAATTGATTGTCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGCAGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCTATTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15602.88 Da
• Isoelectric Point: 5.6780

>AT197773 WP_219247917.1 NZ_CP072453:1458558-1458974 [Providencia sp. R33]
MIINDAVKAANSLINIVPLLGGSHSRKDYDEAIKLVEYLVEHDPDNPLIDMICAKIDLYEENAPEFKAFNARLNSCDDAV
AVLRTLMDQYKLNTTDFKNELGSRSYVSRILNGERNLTLDHMKKLASRFGLPVSTFIR

Nucleotide

• Length: 417 bp

>AT197773 NZ_CP096698:448382-448639 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ACCCTGATTTACTAGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA