Detailed information of TA system TA197760

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3870809..3871406
• Replicon: chromosome
• Accession: NZ_CP072422
• Organism: Klebsiella pneumoniae strain B33769

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: J6255_RS18700
• Protein ID: WP_004893639.1
• Coordinates: 3871089..3871406 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: J6255_RS18695
• Protein ID: WP_004142561.1
• Coordinates: 3870809..3871096 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6255_RS18665 (J6255_18660)	3866889..3867137	+	249	WP_002893037.1	DUF1158 domain-containing protein	-
J6255_RS18670 (J6255_18665)	3867155..3867496	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
J6255_RS18675 (J6255_18670)	3867527..3868642	-	1116	WP_012737592.1	MBL fold metallo-hydrolase	-
J6255_RS18680 (J6255_18675)	3868822..3869403	+	582	WP_004176968.1	TetR/AcrR family transcriptional regulator	-
J6255_RS18685 (J6255_18680)	3869403..3869771	+	369	WP_004142557.1	MmcQ/YjbR family DNA-binding protein	-
J6255_RS18690 (J6255_18685)	3869891..3870544	+	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
J6255_RS18695 (J6255_18690)	3870809..3871096	-	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
J6255_RS18700 (J6255_18695)	3871089..3871406	-	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J6255_RS18705 (J6255_18700)	3871591..3872634	-	1044	WP_004893645.1	DUF2157 domain-containing protein	-
J6255_RS18710 (J6255_18705)	3873300..3874166	-	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
J6255_RS18715 (J6255_18710)	3874275..3875702	+	1428	WP_004176980.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T197760 WP_004893639.1 NZ_CP072422:c3871406-3871089 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T197760 NZ_CP096684:1220604-1220786 [Acinetobacter baumannii]
GTGAAAAGTCTGGATTTAATCAAAATGATTGAAGCAGACGGTTGGTATGAGGTTAGGGTTTCAGGAAGTCATCATCACTT
CAAACACCCAACCAAAAAGGGATTAGTAACAATCCCTCATCCTAAAAAGGATTTACCAAACGGAACTGTTAAAAGCATTT
TGAAGCAAGCGGGTCTAAATTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT197760 WP_004142561.1 NZ_CP072422:c3871096-3870809 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT197760 NZ_CP096684:1220851-1221255 [Acinetobacter baumannii]
ATGTTATATCCAATTGCTATTGAAAGAGGTACAGACACCGAAGCCTTTGGTGTCTCCGTTCCAGATATTCCAGGGTGTTT
CAGCGCAGGCGATACATTATATGAAGCTATCGAGAATGTTAAAGAGGCTATTTCTGGCCACTTGGAAATCCTAGCAGAAG
ATGGAGAGGAGATTCCTTTAGCATCTGATGTCAGTAAGTTTATTGACCAAGAAGATTATAGAGGTATGATCTGGGCAGTT
ACTGAAGTTGATGTTAGCCGTTACTTAGGTAAACCAGAAAAAATCAATGTTACTTTACCAAGCCGATTAATTCGGAAGAT
TGATGATAATGTTGGTAAAGATAAAAGATTTAAAACTCGCTCTGCTTTTTTGGCCGCTGGTGCTGAAAAGCTACTACATG
CTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1