Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 357797..358443 Replicon chromosome
Accession NZ_CP072422
Organism Klebsiella pneumoniae strain B33769

Toxin (Protein)


Gene name higB Uniprot ID A0A483PC41
Locus tag J6255_RS01650 Protein ID WP_004216295.1
Coordinates 357797..358144 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag J6255_RS01655 Protein ID WP_002920557.1
Coordinates 358144..358443 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6255_RS01640 (J6255_01640) 353723..355156 + 1434 WP_004889438.1 glycogen synthase GlgA -
J6255_RS01645 (J6255_01645) 355174..357621 + 2448 WP_134919826.1 glycogen phosphorylase -
J6255_RS01650 (J6255_01650) 357797..358144 + 348 WP_004216295.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
J6255_RS01655 (J6255_01655) 358144..358443 + 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
J6255_RS01660 (J6255_01660) 358506..360014 - 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
J6255_RS01665 (J6255_01665) 360219..360548 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
J6255_RS01670 (J6255_01670) 360599..361429 + 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
J6255_RS01675 (J6255_01675) 361479..362237 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13606.61 Da        Isoelectric Point: 5.6749

>T197748 WP_004216295.1 NZ_CP072422:357797-358144 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCADNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T197748 NZ_CP096666:3503941-3504237 [Yersinia pestis EV76-CN]
ATGGAGAAACGGACTCCGCATACTCGTTTATTAAAAGTAAAAGAATTGGTATTGAAGGGGAATATTAAAACGACACGAAC
TGCCAGAGATGGGGCTGAGGAACTGGGACTGAGTTTTAGAGATATGTGTGATGCAGTTTCCGAACTGATTAGCGCCGATT
TTTATAAAAGTATGACGACACATCAAGATCATACCATTTGGCAGGATGTTTACCGTCCTATGCTTTCTTGCGGTAGAGTT
TATTTGAAAATCACGGTTATCGATGATGTTTTGAATGTCTCGTTTAAGGAGATTTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT197748 WP_002920557.1 NZ_CP072422:358144-358443 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT197748 NZ_CP096666:3504237-3504635 [Yersinia pestis EV76-CN]
ATGATGAAATGTCCAGTTTGTGGCGGTGTAGAATTAATCCACGAAGCGCGTGACGTTCCCTTTACCTATAAAGGGCGTAA
GTTCATCGTCGAAGGCGTGATAGGGCAACATTGCCCGGCATGCGGTGAAACAGTCATGACCGAAGCCGAGTCCGATGTCT
ATGCAGCAAAAACAATGCTTTTGCGAAAAATGGTCAATACAGAATCGATTGCCCCCGCATATATTGTGCAGATCCGAAAA
AAGTTGGGCCTAAACCAGCGGGAAGCCAGTGAGATTTTTGGTGGTGGGGTTAATGCATTTTCGCGTTACGAAAAAGGTAA
GGCTTTGCCACATCCATCAACGATAAAACTGTTGCAAGTGCTTGATAAACACCCGGAGTTATTGAATGAAATTAGGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483PC41

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References