Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 331610..332196 Replicon chromosome
Accession NZ_CP072422
Organism Klebsiella pneumoniae strain B33769

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag J6255_RS01540 Protein ID WP_002920800.1
Coordinates 331828..332196 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag J6255_RS01535 Protein ID WP_004174006.1
Coordinates 331610..331831 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6255_RS01515 (J6255_01515) 327767..328693 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
J6255_RS01520 (J6255_01520) 328690..329967 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
J6255_RS01525 (J6255_01525) 329964..330731 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
J6255_RS01530 (J6255_01530) 330733..331446 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
J6255_RS01535 (J6255_01535) 331610..331831 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
J6255_RS01540 (J6255_01540) 331828..332196 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
J6255_RS01545 (J6255_01545) 332469..333785 + 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
J6255_RS01550 (J6255_01550) 333892..334779 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
J6255_RS01555 (J6255_01555) 334776..335621 + 846 WP_004185988.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
J6255_RS01560 (J6255_01560) 335623..336693 + 1071 WP_017880090.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 328690..337430 8740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T197747 WP_002920800.1 NZ_CP072422:331828-332196 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T197747 NZ_CP096666:3489531-3489956 [Yersinia pestis EV76-CN]
GTGGCCCAGTGGCGATGTAACCTGCGGGTATCCTGGCATACTCAACTCTTTTCTTTATTGGCACACGGCATATTGGTCAT
TTTAACCTTGGTCGCGCCCTGGCCCTTGGGTTATACCGCCCTATGGCTGGTATTGCTGACGTTAGTGGTGTTTGAGTGTA
TCCGCAGCCAGAAAAGGATTAAATCCTGTCAGGGGGAAATCAGGCTAAAACCTGGCAACCTGGTGCGATGGAAAAGGCAT
GAATGGACCGTGGTCAAGCCGCCGTGGATCACCCGCTATGGGGTATTATTACATTTACAACAAACAGGCGGCCACGCGAC
CCGAAAGCGTCTATGGCTGTCCGCTGACAGCATGTCTGAAGATGAGTGGCGGCAGCTATGCCTGTTGCTGCGGCATTCAT
TTGAATCTGACGATGGAACGATGTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT197747 WP_004174006.1 NZ_CP072422:331610-331831 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT197747 NZ_CP096666:3489284-3489550 [Yersinia pestis EV76-CN]
ATGGAAATTGATAATAAAGCCCGTATTCACTGGGCATGCCGCCGTGGTATGCGTGAACTTGATATCTCCATCATGCCGTT
TTTTGAATATGAATATGATAGCTTGAGCGATAACGAGAAACAGGCATTTATCCGCTTGCTTGAGTGTGATGACCCAGATT
TATTCAACTGGCTGATGAACCACGGGGAGCCGCAGGATAGCGAGCTATATCAGATGGTCAAACTGATTCAATCACGAAAT
AAAGCCCGTGGCCCAGTGGCGATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References