Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3873474..3874071 Replicon chromosome
Accession NZ_CP072413
Organism Klebsiella pneumoniae strain B32278

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag J6256_RS18930 Protein ID WP_004142563.1
Coordinates 3873754..3874071 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag J6256_RS18925 Protein ID WP_004142561.1
Coordinates 3873474..3873761 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6256_RS18895 (3869569) 3869569..3869817 + 249 WP_002893037.1 DUF1158 domain-containing protein -
J6256_RS18900 (3869835) 3869835..3870176 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
J6256_RS18905 (3870207) 3870207..3871322 - 1116 WP_012737592.1 MBL fold metallo-hydrolase -
J6256_RS18910 (3871502) 3871502..3872086 + 585 WP_071579668.1 TetR/AcrR family transcriptional regulator -
J6256_RS18915 (3872083) 3872083..3872451 + 369 WP_040171587.1 MmcQ/YjbR family DNA-binding protein -
J6256_RS18920 (3872571) 3872571..3873209 + 639 WP_064277739.1 oxygen-insensitive NAD(P)H nitroreductase -
J6256_RS18925 (3873474) 3873474..3873761 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
J6256_RS18930 (3873754) 3873754..3874071 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
J6256_RS18935 (3874256) 3874256..3875299 - 1044 WP_040153598.1 DUF2157 domain-containing protein -
J6256_RS18940 (3875969) 3875969..3876835 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
J6256_RS18945 (3876944) 3876944..3878371 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T197742 WP_004142563.1 NZ_CP072413:c3874071-3873754 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T197742 NZ_CP096665:c4994201-4993923 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT197742 WP_004142561.1 NZ_CP072413:c3873761-3873474 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT197742 NZ_CP096665:c4993911-4993606 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References