Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3882224..3882821 Replicon chromosome
Accession NZ_CP072403
Organism Klebsiella pneumoniae strain B13243

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag J6322_RS18995 Protein ID WP_004142563.1
Coordinates 3882504..3882821 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag J6322_RS18990 Protein ID WP_004142561.1
Coordinates 3882224..3882511 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6322_RS18960 (3878319) 3878319..3878567 + 249 WP_002893037.1 DUF1158 domain-containing protein -
J6322_RS18965 (3878585) 3878585..3878926 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
J6322_RS18970 (3878957) 3878957..3880072 - 1116 WP_012737592.1 MBL fold metallo-hydrolase -
J6322_RS18975 (3880252) 3880252..3880836 + 585 WP_071579668.1 TetR/AcrR family transcriptional regulator -
J6322_RS18980 (3880833) 3880833..3881201 + 369 WP_040171587.1 MmcQ/YjbR family DNA-binding protein -
J6322_RS18985 (3881321) 3881321..3881959 + 639 WP_064277739.1 oxygen-insensitive NAD(P)H nitroreductase -
J6322_RS18990 (3882224) 3882224..3882511 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
J6322_RS18995 (3882504) 3882504..3882821 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
J6322_RS19000 (3883006) 3883006..3884049 - 1044 WP_040153598.1 DUF2157 domain-containing protein -
J6322_RS19005 (3884719) 3884719..3885585 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
J6322_RS19010 (3885694) 3885694..3887121 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T197710 WP_004142563.1 NZ_CP072403:c3882821-3882504 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T197710 NZ_CP096587:c4170751-4170401 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT197710 WP_004142561.1 NZ_CP072403:c3882511-3882224 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT197710 NZ_CP096587:c4170996-4170745 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References