Detailed information of TA system TA197698

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2754..3405
• Replicon: plasmid p3KSK2
• Accession: NZ_CP072401
• Organism: Acinetobacter baumannii strain KSK2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A4R0DYU1
• Locus tag: J6B58_RS21135
• Protein ID: WP_000269907.1
• Coordinates: 2754..3110 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J6B58_RS21140
• Protein ID: WP_001140617.1
• Coordinates: 3103..3405 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6B58_RS21120 (J6B58_21120)	1..951	+	951	WP_001205343.1	replication initiation protein RepM	-
J6B58_RS21125 (J6B58_21125)	944..1519	+	576	WP_001096616.1	plasmid replication DNA-binding protein	-
J6B58_RS21130 (J6B58_21130)	1539..1682	+	144	WP_001125244.1	hypothetical protein	-
J6B58_RS21385	1867..2070	-	204	WP_000482656.1	hypothetical protein	-
J6B58_RS21135 (J6B58_21135)	2754..3110	+	357	WP_000269907.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J6B58_RS21140 (J6B58_21140)	3103..3405	+	303	WP_001140617.1	XRE family transcriptional regulator	Antitoxin
J6B58_RS21145 (J6B58_21145)	3528..3929	+	402	WP_000660211.1	hypothetical protein	-
J6B58_RS21155 (J6B58_21155)	4313..5053	-	741	WP_031380848.1	DUF3800 domain-containing protein	-
J6B58_RS21160 (J6B58_21160)	5364..5750	+	387	WP_001986792.1	helix-turn-helix domain-containing protein	-
J6B58_RS21165 (J6B58_21165)	5787..5960	-	174	WP_001282484.1	hypothetical protein	-
J6B58_RS21170 (J6B58_21170)	5960..6331	-	372	WP_000504218.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..7540	7540

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13488.42 Da
• Isoelectric Point: 5.7172

>T197698 WP_000269907.1 NZ_CP072401:2754-3110 [Acinetobacter baumannii]
MWTVITTDLFNEWLEQQDESTQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKGGKKRFYTEMLAIADQQYELHLSTLGEQSNG

Nucleotide

• Length: 357 bp

>T197698 NZ_CP096587:984086-984421 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11262.05 Da
• Isoelectric Point: 5.8961

>AT197698 WP_001140617.1 NZ_CP072401:3103-3405 [Acinetobacter baumannii]
MAKTLQELLANRSPESQARIQKIADELLLENQLYLIREELEISQKELAETLGIKQPSLSAIENRGNDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT197698 NZ_CP096587:983838-984086 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A4R0DYU1