Detailed information of TA system TA197697

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 22237..22895
• Replicon: plasmid p2KSK2
• Accession: NZ_CP072400
• Organism: Acinetobacter baumannii strain KSK2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: J6B58_RS20765
• Protein ID: WP_000312250.1
• Coordinates: 22536..22895 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J6B58_RS20760
• Protein ID: WP_001096429.1
• Coordinates: 22237..22536 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6B58_RS20720 (J6B58_20720)	17261..18541	+	1281	WP_162200943.1	hypothetical protein	-
J6B58_RS20725 (J6B58_20725)	18646..18903	+	258	WP_000834290.1	hypothetical protein	-
J6B58_RS20730 (J6B58_20730)	18908..19480	+	573	WP_000443897.1	hypothetical protein	-
J6B58_RS20735 (J6B58_20735)	19456..19635	+	180	WP_000387630.1	hypothetical protein	-
J6B58_RS20740 (J6B58_20740)	19652..20281	+	630	WP_000701003.1	hypothetical protein	-
J6B58_RS20745 (J6B58_20745)	20321..20539	+	219	WP_001043201.1	hypothetical protein	-
J6B58_RS20750 (J6B58_20750)	20559..21113	+	555	WP_000790084.1	hypothetical protein	-
J6B58_RS20755 (J6B58_20755)	21163..21699	+	537	WP_000731978.1	hypothetical protein	-
J6B58_RS20760 (J6B58_20760)	22237..22536	-	300	WP_001096429.1	XRE family transcriptional regulator	Antitoxin
J6B58_RS20765 (J6B58_20765)	22536..22895	-	360	WP_000312250.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J6B58_RS20770 (J6B58_20770)	23096..23662	+	567	WP_000710385.1	hypothetical protein	-
J6B58_RS20775 (J6B58_20775)	23711..23893	+	183	WP_000373385.1	hypothetical protein	-
J6B58_RS20780 (J6B58_20780)	23960..24658	+	699	WP_000873188.1	hypothetical protein	-
J6B58_RS20785 (J6B58_20785)	24797..25180	+	384	WP_000654348.1	hypothetical protein	-
J6B58_RS20790 (J6B58_20790)	25247..26005	+	759	WP_001053127.1	hypothetical protein	-
J6B58_RS20795 (J6B58_20795)	27061..27375	+	315	WP_000708714.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaOXA-23 / aph(3')-VIa	-	1..87529	87529

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-88)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13751.62 Da
• Isoelectric Point: 4.8212

>T197697 WP_000312250.1 NZ_CP072400:c22895-22536 [Acinetobacter baumannii]
MAWDVETTELFDSWLAEQDENAQDKILASLLVLSELGPNLGRPHVDTIKESKYPNMKEIRVQVKGHPIRGFFAFDPERKA
IVLCAGDKKGLNEKAFYKEMIKIADEQYEQYLRDNYGDK

Nucleotide

• Length: 360 bp

>T197697 NZ_CP096587:861417-861824 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11012.93 Da
• Isoelectric Point: 10.4856

>AT197697 WP_001096429.1 NZ_CP072400:c22536-22237 [Acinetobacter baumannii]
MAKLNDIISKRSPESQQRIKKMANQMLLDINLSNLREELKISQAEIAKNLGVSQAAISKREKLGKDLKISSLKEYVEAMG
GKVRLDVELPNGKHVAISI

Nucleotide

• Length: 300 bp

>AT197697 NZ_CP096587:861170-861436 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA