Detailed information of TA system TA197673

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 604580..605171
• Replicon: chromosome
• Accession: NZ_CP072385
• Organism: Arachnia propionica strain F0714

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A4V1GCM2
• Locus tag: J5A53_RS02635
• Protein ID: WP_014847602.1
• Coordinates: 604580..604861 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J5A53_RS02640
• Protein ID: WP_014847601.1
• Coordinates: 604872..605171 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5A53_RS02615 (J5A53_02615)	599724..600023	-	300	WP_041696711.1	helix-turn-helix transcriptional regulator	-
J5A53_RS02620 (J5A53_02620)	600023..600382	-	360	WP_014847604.1	type II toxin-antitoxin system RelE/ParE family toxin	-
J5A53_RS02625 (J5A53_02625)	601178..602977	-	1800	WP_051015059.1	tetratricopeptide repeat protein	-
J5A53_RS02630 (J5A53_02630)	602974..604275	-	1302	WP_014847603.1	hypothetical protein	-
J5A53_RS02635 (J5A53_02635)	604580..604861	+	282	WP_014847602.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J5A53_RS02640 (J5A53_02640)	604872..605171	+	300	WP_014847601.1	HigA family addiction module antitoxin	Antitoxin
J5A53_RS02645 (J5A53_02645)	605216..605725	+	510	WP_014847600.1	GNAT family N-acetyltransferase	-
J5A53_RS02650 (J5A53_02650)	606110..606262	+	153	WP_014847599.1	hypothetical protein	-
J5A53_RS02655 (J5A53_02655)	606887..607756	+	870	WP_051015058.1	DUF4839 domain-containing protein	-
J5A53_RS02660 (J5A53_02660)	607878..609107	-	1230	WP_014847598.1	ATP-binding protein	-
J5A53_RS02665 (J5A53_02665)	609276..609500	+	225	WP_014847597.1	toxin-antitoxin system toxin component PIN family	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (5-80)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10976.73 Da
• Isoelectric Point: 10.0040

>T197673 WP_014847602.1 NZ_CP072385:604580-604861 [Arachnia propionica]
MIVSFADRDAERLFQRQRVKRLDPRLHRKALIKLWLLDAVHELDELRVPPGNRLEALKGARIGQHSIRINDQWRICFVWT
PAGPSEVEIVDYH

Nucleotide

• Length: 282 bp

>T197673 NZ_CP096567:122125-122424 [Rhodococcus qingshengii JCM 15477]
ATGCGACCAATTCACGAAGCCAAGCTCGACACAGTTCGGCCGGTCCTCGTACTCACCCGAGAGATGGTCCGTCCACACCT
CGGGACCGTCACGGTAGCGCCCATCACCGGCGAGATCCGAGGACTGTCCACTGAGGTGTCCGTCAGCGTCGCTAACGGAC
TATCCGCCGACGCGGTGGTCAGTTGCGACCACATCACCACCATTCCAGTCAGTGCACTCGGCAACATCATCGGTTATCTT
CTTCCAGCGCAAGAGGAATCACTGTCCATAGCCGTGCGCGCCGCCTTCGATCTCGAATGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11197.96 Da
• Isoelectric Point: 7.4040

>AT197673 WP_014847601.1 NZ_CP072385:604872-605171 [Arachnia propionica]
MDNKMAPVHPGEILVEEFLNPMGVTQHRLAISIGVPPRRINEIVHGKRRITADTALRLGRFFGMNPQFWVNLQSHYDLEI
GKDALGDELDKIQPLRSVA

Nucleotide

• Length: 300 bp

>AT197673 NZ_CP096567:121889-122125 [Rhodococcus qingshengii JCM 15477]
ATGAGCACGCAGATCGCGGTACGACTACCGGACGAGATCGTGGAATTTCTCGACGACGAAGTCAGGGAACATCGCGCCAC
CTCGCGAGCCTCACTAGTGTTGCGCGCTCTCGAACGGGAGCGTCGCCGTCAGATAGCCGCACGAGACGCGGAGATCCTCT
CGGCCGCAACATCCGAACATGATTTGAGCCCCCTCGCTCACCACGCCGTGAATCGACAGCCAACAGGCTCGACCTGA

Structures

Toxin

• AlphaFold DB: A0A4V1GCM2