Detailed information of TA system TA197672

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 599724..600382
• Replicon: chromosome
• Accession: NZ_CP072385
• Organism: Arachnia propionica strain F0714

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: A0A4P8ZSU4
• Locus tag: J5A53_RS02620
• Protein ID: WP_014847604.1
• Coordinates: 600023..600382 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: HigA2
• Uniprot ID: -
• Locus tag: J5A53_RS02615
• Protein ID: WP_041696711.1
• Coordinates: 599724..600023 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5A53_RS02595 (J5A53_02595)	595142..596299	+	1158	WP_041696714.1	DNA (cytosine-5-)-methyltransferase	-
J5A53_RS02600 (J5A53_02600)	596355..597428	+	1074	WP_014847607.1	restriction endonuclease, SacI family	-
J5A53_RS15790 (J5A53_02605)	597730..598011	-	282	WP_041696713.1	helix-turn-helix domain-containing protein	-
J5A53_RS02610 (J5A53_02610)	598584..599339	+	756	WP_014847606.1	transposase family protein	-
J5A53_RS02615 (J5A53_02615)	599724..600023	-	300	WP_041696711.1	helix-turn-helix transcriptional regulator	Antitoxin
J5A53_RS02620 (J5A53_02620)	600023..600382	-	360	WP_014847604.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J5A53_RS02625 (J5A53_02625)	601178..602977	-	1800	WP_051015059.1	tetratricopeptide repeat protein	-
J5A53_RS02630 (J5A53_02630)	602974..604275	-	1302	WP_014847603.1	hypothetical protein	-
J5A53_RS02635 (J5A53_02635)	604580..604861	+	282	WP_014847602.1	type II toxin-antitoxin system RelE/ParE family toxin	-
J5A53_RS02640 (J5A53_02640)	604872..605171	+	300	WP_014847601.1	HigA family addiction module antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (11-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-86)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13799.04 Da
• Isoelectric Point: 10.7132

>T197672 WP_014847604.1 NZ_CP072385:c600382-600023 [Arachnia propionica]
MWEVDVTLIADWLRSLDQTSYEQVIAALEVLRDRGLSLGRPLVDTITASRHRNMKELRPGSIGRSEVRILFAFDPKRMAI
LLIAGDKAGSWTRWYKRNVPLADDLFDKHIRALRKRPEK

Nucleotide

• Length: 360 bp

>T197672 NZ_CP096540:c2269420-2269211 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATTGATGGTCAAGTAACGGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATTTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11031.61 Da
• Isoelectric Point: 7.2036

>AT197672 WP_041696711.1 NZ_CP072385:c600023-599724 [Arachnia propionica]
MTIDDILAERSVDRSQVEAHKARMLAEVRAYRLRELRENAGLTQAQLAERIGVGQRQISKIEHGDLDTAKIGTIRRYLEA
IGGSLSLEYVAGDLKVVIN

Nucleotide

• Length: 300 bp

>AT197672 NZ_CP096540:c2269725-2269432 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGAGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• AlphaFold DB: A0A4P8ZSU4