Detailed information of TA system TA197664

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2095201..2095756
• Replicon: chromosome
• Accession: NZ_CP072380
• Organism: Atopobium sp. oral taxon 416 strain W9116

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: J4859_RS10965
• Protein ID: WP_212329806.1
• Coordinates: 2095201..2095485 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J4859_RS10970
• Protein ID: WP_249113611.1
• Coordinates: 2095478..2095756 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J4859_RS10945 (J4859_10940)	2090771..2091784	+	1014	WP_212329800.1	AraC family transcriptional regulator	-
J4859_RS10950 (J4859_10945)	2092587..2093480	-	894	Protein_2150	transposase	-
J4859_RS10960 (J4859_10955)	2093934..2094893	-	960	WP_249113610.1	haloacid dehalogenase-like hydrolase	-
J4859_RS10965 (J4859_10960)	2095201..2095485	+	285	WP_212329806.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J4859_RS10970 (J4859_10965)	2095478..2095756	+	279	WP_249113611.1	HigA family addiction module antitoxin	Antitoxin
J4859_RS17035	2096069..2096203	-	135	WP_256436725.1	hypothetical protein	-
J4859_RS10975 (J4859_10970)	2096239..2096694	-	456	Protein_2155	transposase	-
J4859_RS17040	2096948..2097073	-	126	WP_256436726.1	hypothetical protein	-
J4859_RS10985 (J4859_10980)	2097335..2097481	-	147	WP_212329812.1	hypothetical protein	-
J4859_RS10990 (J4859_10985)	2097830..2098327	+	498	WP_212329814.1	hypothetical protein	-
J4859_RS10995 (J4859_10990)	2098527..2098829	+	303	WP_212329815.1	helix-turn-helix domain-containing protein	-
J4859_RS11000 (J4859_10995)	2099085..2100077	+	993	WP_249113612.1	HipA domain-containing protein	-
J4859_RS11005 (J4859_11000)	2100167..2100724	-	558	WP_212329817.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (15-66)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11111.71 Da
• Isoelectric Point: 9.2011

>T197664 WP_212329806.1 NZ_CP072380:2095201-2095485 [Atopobium sp. oral taxon 416]
MEFRDADIRDFWEGTNFTPRRVPSDVRRALMRKLQILDAACVLDDLKVPPGNRLEALAGDRKGQYSIRVNRRWRLCFKWS
NGEAKEVEFVDYHV

Nucleotide

• Length: 285 bp

>T197664 NZ_CP096539:c2123135-2122773 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAACAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTAAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10175.95 Da
• Isoelectric Point: 4.5510

>AT197664 WP_249113611.1 NZ_CP072380:2095478-2095756 [Atopobium sp. oral taxon 416]
MCDLMSIPGEILKEEFLEPLGISQYRLAKAIGKSESAVSEIVNGKRVITVEMAWLLAEALSTTPEFWMKLQTTYDLKTFD
PSALPKVTALAV

Nucleotide

• Length: 279 bp

>AT197664 NZ_CP096539:c2123302-2123132 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA