Detailed information of TA system TA197650

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 114581..115007
• Replicon: plasmid unnamed1
• Accession: NZ_CP072323
• Organism: Escherichia coli strain EC-JS426

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: J6247_RS23645
• Protein ID: WP_001372321.1
• Coordinates: 114581..114706 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	114783..115007 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6247_RS23615 (109951)	109951..110640	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
J6247_RS23620 (110827)	110827..111210	-	384	WP_001151524.1	conjugal transfer relaxosome DNA-binding protein TraM	-
J6247_RS23625 (111543)	111543..112133	+	591	WP_000252680.1	transglycosylase SLT domain-containing protein	-
J6247_RS23630 (112430)	112430..113251	-	822	WP_001234469.1	DUF932 domain-containing protein	-
J6247_RS23635 (113372)	113372..113659	-	288	WP_000107535.1	hypothetical protein	-
J6247_RS23640 (113684)	113684..113890	-	207	WP_000547939.1	hypothetical protein	-
J6247_RS24555 (113960)	113960..114133	+	174	Protein_126	hypothetical protein	-
J6247_RS24560 (114131)	114131..114361	-	231	WP_001426396.1	hypothetical protein	-
J6247_RS23645 (114581)	114581..114706	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
J6247_RS24565 (114648)	114648..114797	-	150	Protein_129	plasmid maintenance protein Mok	-
- (114783)	114783..115007	-	225	NuclAT_0	-	Antitoxin
- (114783)	114783..115007	-	225	NuclAT_0	-	Antitoxin
- (114783)	114783..115007	-	225	NuclAT_0	-	Antitoxin
- (114783)	114783..115007	-	225	NuclAT_0	-	Antitoxin
J6247_RS23650 (114819)	114819..115007	+	189	WP_001299721.1	hypothetical protein	-
J6247_RS23655 (114976)	114976..115738	-	763	Protein_131	plasmid SOS inhibition protein A	-
J6247_RS23660 (115735)	115735..116169	-	435	WP_000845895.1	conjugation system SOS inhibitor PsiB	-
J6247_RS23665 (116224)	116224..118182	-	1959	WP_054444128.1	ParB/RepB/Spo0J family partition protein	-
J6247_RS23670 (118241)	118241..118474	-	234	WP_000006004.1	DUF905 domain-containing protein	-
J6247_RS23675 (118530)	118530..119057	-	528	WP_000290793.1	single-stranded DNA-binding protein	-
J6247_RS24680 (119700)	119700..119831	-	132	WP_283570452.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / floR / aph(6)-Id / aph(3'')-Ib / aph(3')-Ia / ant(3'')-Ia / sul3 / aadA2 / blaTEM-1B / mph(A) / dfrA14 / sitABCD	iroB / iroC / iroD / iroE / iroN	1..142200	142200

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T197650 WP_001372321.1 NZ_CP072323:c114706-114581 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T197650 NZ_CP096530:c2163141-2162779 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAACAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTAAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT197650 NZ_CP072323:c115007-114783 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin