Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3700831..3701630 Replicon chromosome
Accession NZ_CP072322
Organism Escherichia coli strain EC-JS426

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag J6247_RS17705 Protein ID WP_000347267.1
Coordinates 3700831..3701295 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag J6247_RS17710 Protein ID WP_001307405.1
Coordinates 3701295..3701630 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6247_RS17675 (3695832) 3695832..3696266 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
J6247_RS17680 (3696284) 3696284..3697162 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
J6247_RS17685 (3697152) 3697152..3697931 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
J6247_RS17690 (3697942) 3697942..3698415 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
J6247_RS17695 (3698438) 3698438..3699718 - 1281 WP_000681933.1 tagatose-bisphosphate aldolase subunit KbaZ -
J6247_RS17700 (3699967) 3699967..3700776 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
J6247_RS17705 (3700831) 3700831..3701295 - 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
J6247_RS17710 (3701295) 3701295..3701630 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
J6247_RS17715 (3701779) 3701779..3703350 - 1572 WP_001273741.1 galactarate dehydratase -
J6247_RS17720 (3703725) 3703725..3705059 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
J6247_RS17725 (3705075) 3705075..3705845 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T197644 WP_000347267.1 NZ_CP072322:c3701295-3700831 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T197644 NZ_CP096528:c1948715-1948563 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACTGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATTTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGACAGTCAGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT197644 WP_001307405.1 NZ_CP072322:c3701630-3701295 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT197644 NZ_CP096528:c1949339-1948740 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTACTAGATGAATTAAAGAAAAATGTAG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAACAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACTGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTTGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
CGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References