Detailed information of TA system TA197642

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2888501..2889103
• Replicon: chromosome
• Accession: NZ_CP072322
• Organism: Escherichia coli strain EC-JS426

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: J6247_RS13795
• Protein ID: WP_000897305.1
• Coordinates: 2888792..2889103 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J6247_RS13790
• Protein ID: WP_000356397.1
• Coordinates: 2888501..2888791 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6247_RS13765 (2884427)	2884427..2885329	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
J6247_RS13770 (2885326)	2885326..2885961	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
J6247_RS13775 (2885958)	2885958..2886887	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
J6247_RS13780 (2887217)	2887217..2887459	-	243	WP_001086388.1	protein YiiF	-
J6247_RS13785 (2887678)	2887678..2887896	-	219	WP_001297075.1	CopG family transcriptional regulator	-
J6247_RS13790 (2888501)	2888501..2888791	-	291	WP_000356397.1	NadS family protein	Antitoxin
J6247_RS13795 (2888792)	2888792..2889103	-	312	WP_000897305.1	hypothetical protein	Toxin
J6247_RS13800 (2889332)	2889332..2890240	+	909	WP_001162704.1	alpha/beta hydrolase	-
J6247_RS13805 (2890304)	2890304..2891245	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
J6247_RS13810 (2891290)	2891290..2891727	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
J6247_RS13815 (2891724)	2891724..2892596	-	873	WP_072643613.1	virulence factor BrkB family protein	-
J6247_RS13820 (2892590)	2892590..2893189	-	600	WP_001295269.1	glucose-1-phosphatase	-
J6247_RS13825 (2893288)	2893288..2894073	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T197642 WP_000897305.1 NZ_CP072322:c2889103-2888792 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T197642 NZ_CP096526:c2143925-2143716 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATTGATGGTCAAGTAACGGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATTTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT197642 WP_000356397.1 NZ_CP072322:c2888791-2888501 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT197642 NZ_CP096526:c2144230-2143937 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGAGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7