Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2032693..2033234 Replicon chromosome
Accession NZ_CP072322
Organism Escherichia coli strain EC-JS426

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag J6247_RS09835 Protein ID WP_000615976.1
Coordinates 2032956..2033234 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag J6247_RS09830 Protein ID WP_000729704.1
Coordinates 2032693..2032953 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6247_RS09805 (2028476) 2028476..2029261 - 786 WP_000207568.1 putative lateral flagellar export/assembly protein LafU -
J6247_RS09810 (2029233) 2029233..2030945 + 1713 Protein_1942 flagellar biosynthesis protein FlhA -
J6247_RS09815 (2031050) 2031050..2031328 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
J6247_RS09820 (2031321) 2031321..2031677 + 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
J6247_RS09825 (2031734) 2031734..2032507 - 774 WP_000543895.1 C40 family peptidase -
J6247_RS09830 (2032693) 2032693..2032953 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
J6247_RS09835 (2032956) 2032956..2033234 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
J6247_RS09840 (2033390) 2033390..2034130 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
J6247_RS09845 (2034101) 2034101..2034868 - 768 WP_000333380.1 class II glutamine amidotransferase -
J6247_RS09850 (2035074) 2035074..2035652 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 2031321..2042846 11525


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T197639 WP_000615976.1 NZ_CP072322:2032956-2033234 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T197639 NZ_CP096526:800339-800491 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACTGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATTTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGACAGTCAGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT197639 WP_000729704.1 NZ_CP072322:2032693-2032953 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT197639 NZ_CP096526:799715-800314 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTACTAGATGAATTAAAGAAAAATGTAG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAACAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACTGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTTGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
CGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References