Detailed information of TA system TA197603

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3823..4481
• Replicon: plasmid p2KSK20
• Accession: NZ_CP072302
• Organism: Acinetobacter baumannii strain KSK20

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: J6I82_RS20680
• Protein ID: WP_000312250.1
• Coordinates: 3823..4182 (+)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J6I82_RS20685
• Protein ID: WP_001096429.1
• Coordinates: 4182..4481 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6I82_RS20655 (J6I82_20655)	713..1471	-	759	WP_001053127.1	hypothetical protein	-
J6I82_RS20660 (J6I82_20660)	1538..1921	-	384	WP_000654348.1	hypothetical protein	-
J6I82_RS20665 (J6I82_20665)	2060..2758	-	699	WP_000873188.1	hypothetical protein	-
J6I82_RS20670 (J6I82_20670)	2825..3007	-	183	WP_000373385.1	hypothetical protein	-
J6I82_RS20675 (J6I82_20675)	3056..3622	-	567	WP_000710385.1	hypothetical protein	-
J6I82_RS20680 (J6I82_20680)	3823..4182	+	360	WP_000312250.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J6I82_RS20685 (J6I82_20685)	4182..4481	+	300	WP_001096429.1	XRE family transcriptional regulator	Antitoxin
J6I82_RS20690 (J6I82_20690)	5019..5555	-	537	WP_000731978.1	hypothetical protein	-
J6I82_RS20695 (J6I82_20695)	5605..6159	-	555	WP_000790084.1	hypothetical protein	-
J6I82_RS20700 (J6I82_20700)	6179..6397	-	219	WP_001043201.1	hypothetical protein	-
J6I82_RS20705 (J6I82_20705)	6437..7066	-	630	WP_000701003.1	hypothetical protein	-
J6I82_RS20710 (J6I82_20710)	7083..7262	-	180	WP_000387630.1	hypothetical protein	-
J6I82_RS20715 (J6I82_20715)	7238..7810	-	573	WP_000443897.1	hypothetical protein	-
J6I82_RS20720 (J6I82_20720)	7815..8072	-	258	WP_000834290.1	hypothetical protein	-
J6I82_RS20725 (J6I82_20725)	8177..9457	-	1281	WP_162200943.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3')-VI	-	1..68309	68309

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-88)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13751.62 Da
• Isoelectric Point: 4.8212

>T197603 WP_000312250.1 NZ_CP072302:3823-4182 [Acinetobacter baumannii]
MAWDVETTELFDSWLAEQDENAQDKILASLLVLSELGPNLGRPHVDTIKESKYPNMKEIRVQVKGHPIRGFFAFDPERKA
IVLCAGDKKGLNEKAFYKEMIKIADEQYEQYLRDNYGDK

Nucleotide

• Length: 360 bp

>T197603 NZ_CP096264:c177022-176738 [Klebsiella quasipneumoniae]
ATGACCTATAAGTTAGCCTTCAATGAATCAGCTTTGAAAGAATGGAAAAAACTGGGTCATACTATTCGGGAGCAATTCAA
AAAGAAGCTTTCCGAACGGCTTCAGAACCCACGGGTGCCCGCAGCACAACTCCATGGAGGTAAAGACCAGTATAAAATAA
AGCTCCATGGCGCGGGTTACCGACTGGTTTACAGTGTCAACGATGAAATTGTGACGGTCACCGTAATTGGGGTGGGCAAA
CGCAACAATGATGATATCTACAACGCAACCCGACACAGAAATTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11012.93 Da
• Isoelectric Point: 10.4856

>AT197603 WP_001096429.1 NZ_CP072302:4182-4481 [Acinetobacter baumannii]
MAKLNDIISKRSPESQQRIKKMANQMLLDINLSNLREELKISQAEIAKNLGVSQAAISKREKLGKDLKISSLKEYVEAMG
GKVRLDVELPNGKHVAISI

Nucleotide

• Length: 300 bp

>AT197603 NZ_CP096264:c177260-177012 [Klebsiella quasipneumoniae]
ATGGCATTTCAGGTTTTAACCACCACAGCAGCCAGCATTACAGAACTTAAACGTGACCCTATGGGTACCTTCAATGCAGG
AGAAGGTGCACCGGTAGCAATCCTGAATCGCAACGAACCCGCTTTTTACTGTGTACCACCAGCCCTGTATGCTCAGCTGA
TGGAAATGCTGGAAGATCAAGAGTTGGGGCGTATTGCTGATGAACGAATCGGTGAGGACCGAATCGAGGTCAACATTGAT
GACCTATAA