Detailed information of TA system TA197597

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 22237..22895
• Replicon: plasmid p2KSK19
• Accession: NZ_CP072297
• Organism: Acinetobacter baumannii strain KSK19

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: J6I63_RS20835
• Protein ID: WP_000312250.1
• Coordinates: 22536..22895 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J6I63_RS20830
• Protein ID: WP_001096429.1
• Coordinates: 22237..22536 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6I63_RS20790 (J6I63_20790)	17261..18541	+	1281	WP_162200943.1	hypothetical protein	-
J6I63_RS20795 (J6I63_20795)	18646..18903	+	258	WP_000834290.1	hypothetical protein	-
J6I63_RS20800 (J6I63_20800)	18908..19480	+	573	WP_000443897.1	hypothetical protein	-
J6I63_RS20805 (J6I63_20805)	19456..19635	+	180	WP_000387630.1	hypothetical protein	-
J6I63_RS20810 (J6I63_20810)	19652..20281	+	630	WP_000701003.1	hypothetical protein	-
J6I63_RS20815 (J6I63_20815)	20321..20539	+	219	WP_001043201.1	hypothetical protein	-
J6I63_RS20820 (J6I63_20820)	20559..21113	+	555	WP_000790084.1	hypothetical protein	-
J6I63_RS20825 (J6I63_20825)	21163..21699	+	537	WP_000731978.1	hypothetical protein	-
J6I63_RS20830 (J6I63_20830)	22237..22536	-	300	WP_001096429.1	XRE family transcriptional regulator	Antitoxin
J6I63_RS20835 (J6I63_20835)	22536..22895	-	360	WP_000312250.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J6I63_RS20840 (J6I63_20840)	23096..23662	+	567	WP_000710385.1	hypothetical protein	-
J6I63_RS20845 (J6I63_20845)	23711..23893	+	183	WP_000373385.1	hypothetical protein	-
J6I63_RS20850 (J6I63_20850)	23960..24658	+	699	WP_000873188.1	hypothetical protein	-
J6I63_RS20855 (J6I63_20855)	24797..25180	+	384	WP_000654348.1	hypothetical protein	-
J6I63_RS20860 (J6I63_20860)	25247..26005	+	759	WP_001053127.1	hypothetical protein	-
J6I63_RS20865 (J6I63_20865)	27061..27375	+	315	WP_000708714.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaOXA-23 / aph(3')-VIa	-	1..87529	87529

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-88)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13751.62 Da
• Isoelectric Point: 4.8212

>T197597 WP_000312250.1 NZ_CP072297:c22895-22536 [Acinetobacter baumannii]
MAWDVETTELFDSWLAEQDENAQDKILASLLVLSELGPNLGRPHVDTIKESKYPNMKEIRVQVKGHPIRGFFAFDPERKA
IVLCAGDKKGLNEKAFYKEMIKIADEQYEQYLRDNYGDK

Nucleotide

• Length: 360 bp

>T197597 NZ_CP096263:395115-395399 [Klebsiella quasipneumoniae]
ATGACCTATGAACTGGCGTTTGATCCGCGTGCCTGGCGCGAATGGCATAAGCTTGGCGAGACGATCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGGTTGTGCAGAATCCGCGAATTGCATCGGCCAGTCTGAGTGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGGGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11012.93 Da
• Isoelectric Point: 10.4856

>AT197597 WP_001096429.1 NZ_CP072297:c22536-22237 [Acinetobacter baumannii]
MAKLNDIISKRSPESQQRIKKMANQMLLDINLSNLREELKISQAEIAKNLGVSQAAISKREKLGKDLKISSLKEYVEAMG
GKVRLDVELPNGKHVAISI

Nucleotide

• Length: 300 bp

>AT197597 NZ_CP096263:394883-395125 [Klebsiella quasipneumoniae]
ATGGCCACGCTCAACGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACGCC
AACGGAGGCGGTGCGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCAGTGAAAAGTATGACTATCAGCGATA
GCGAAGCGGCATTATTACGCACCGTCCGCGAGCGGCTGGAAAACCCGCAGCCGGAGGTGAAGGTAAGTCTGGATGACCTA
TGA