Detailed information of TA system TA197592

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2754..3405
• Replicon: plasmid p3KSK18
• Accession: NZ_CP072293
• Organism: Acinetobacter baumannii strain KSK18

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A4R0DYU1
• Locus tag: J6I51_RS21100
• Protein ID: WP_000269907.1
• Coordinates: 2754..3110 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J6I51_RS21105
• Protein ID: WP_001140617.1
• Coordinates: 3103..3405 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6I51_RS21085 (J6I51_21085)	1..951	+	951	WP_001205343.1	replication initiation protein RepM	-
J6I51_RS21090 (J6I51_21090)	944..1519	+	576	WP_001096616.1	plasmid replication DNA-binding protein	-
J6I51_RS21095 (J6I51_21095)	1539..1682	+	144	WP_001125244.1	hypothetical protein	-
J6I51_RS21345	1867..2070	-	204	WP_000482656.1	hypothetical protein	-
J6I51_RS21100 (J6I51_21100)	2754..3110	+	357	WP_000269907.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J6I51_RS21105 (J6I51_21105)	3103..3405	+	303	WP_001140617.1	XRE family transcriptional regulator	Antitoxin
J6I51_RS21110 (J6I51_21110)	3528..3929	+	402	WP_000660211.1	hypothetical protein	-
J6I51_RS21120 (J6I51_21120)	4313..5053	-	741	WP_031380848.1	DUF3800 domain-containing protein	-
J6I51_RS21125 (J6I51_21125)	5364..5750	+	387	WP_001986792.1	helix-turn-helix domain-containing protein	-
J6I51_RS21130 (J6I51_21130)	5787..5960	-	174	WP_001282484.1	hypothetical protein	-
J6I51_RS21135 (J6I51_21135)	5960..6331	-	372	WP_000504218.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..7540	7540

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13488.42 Da
• Isoelectric Point: 5.7172

>T197592 WP_000269907.1 NZ_CP072293:2754-3110 [Acinetobacter baumannii]
MWTVITTDLFNEWLEQQDESTQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKGGKKRFYTEMLAIADQQYELHLSTLGEQSNG

Nucleotide

• Length: 357 bp

>T197592 NZ_CP096260:c4971943-4971026 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCATCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGCGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11262.05 Da
• Isoelectric Point: 5.8961

>AT197592 WP_001140617.1 NZ_CP072293:3103-3405 [Acinetobacter baumannii]
MAKTLQELLANRSPESQARIQKIADELLLENQLYLIREELEISQKELAETLGIKQPSLSAIENRGNDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT197592 NZ_CP096260:c4972707-4971949 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGGCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A4R0DYU1