Detailed information of TA system TA197543

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4727124..4727726
• Replicon: chromosome
• Accession: NZ_CP072204
• Organism: Escherichia coli strain LWY6

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: J4T81_RS22705
• Protein ID: WP_000897305.1
• Coordinates: 4727415..4727726 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J4T81_RS22700
• Protein ID: WP_000356397.1
• Coordinates: 4727124..4727414 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J4T81_RS22675 (4723049)	4723049..4723951	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
J4T81_RS22680 (4723948)	4723948..4724583	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
J4T81_RS22685 (4724580)	4724580..4725509	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
J4T81_RS22690 (4725839)	4725839..4726081	-	243	WP_001087409.1	protein YiiF	-
J4T81_RS22695 (4726301)	4726301..4726519	-	219	WP_001315930.1	CopG family transcriptional regulator	-
J4T81_RS22700 (4727124)	4727124..4727414	-	291	WP_000356397.1	NadS family protein	Antitoxin
J4T81_RS22705 (4727415)	4727415..4727726	-	312	WP_000897305.1	hypothetical protein	Toxin
J4T81_RS22710 (4727955)	4727955..4728863	+	909	WP_001162704.1	alpha/beta hydrolase	-
J4T81_RS22715 (4728927)	4728927..4729868	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
J4T81_RS22720 (4729913)	4729913..4730350	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
J4T81_RS22725 (4730347)	4730347..4731219	-	873	WP_000920762.1	virulence factor BrkB family protein	-
J4T81_RS22730 (4731213)	4731213..4731812	-	600	WP_001295269.1	glucose-1-phosphatase	-
J4T81_RS22735 (4731911)	4731911..4732696	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T197543 WP_000897305.1 NZ_CP072204:c4727726-4727415 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T197543 NZ_CP096238:c5456107-5455724 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTTGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGGTTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT197543 WP_000356397.1 NZ_CP072204:c4727414-4727124 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT197543 NZ_CP096238:c5456349-5456107 [Klebsiella pneumoniae subsp. pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7