Detailed information of TA system TA197517

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2213173..2213754
• Replicon: chromosome
• Accession: NZ_CP072203
• Organism: Gordonia polyisoprenivorans strain R9

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: J6U32_RS09885
• Protein ID: WP_208795107.1
• Coordinates: 2213473..2213754 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J6U32_RS09880
• Protein ID: WP_208795105.1
• Coordinates: 2213173..2213457 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6U32_RS09860 (J6U32_09860)	2208872..2210068	+	1197	WP_244332913.1	MazG family protein	-
J6U32_RS09865 (J6U32_09865)	2210089..2210814	-	726	WP_244332766.1	GntR family transcriptional regulator	-
J6U32_RS09870 (J6U32_09870)	2210924..2211607	-	684	WP_208795101.1	phosphonatase-like hydrolase	-
J6U32_RS09875 (J6U32_09875)	2211643..2212788	-	1146	WP_208795103.1	TIGR03364 family FAD-dependent oxidoreductase	-
J6U32_RS09880 (J6U32_09880)	2213173..2213457	-	285	WP_208795105.1	HigA family addiction module antitoxin	Antitoxin
J6U32_RS09885 (J6U32_09885)	2213473..2213754	-	282	WP_208795107.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J6U32_RS09890 (J6U32_09890)	2213857..2214495	+	639	WP_208795109.1	GntR family transcriptional regulator	-
J6U32_RS09895 (J6U32_09895)	2214492..2215748	+	1257	WP_208795111.1	MFS transporter	-
J6U32_RS09900 (J6U32_09900)	2215774..2216661	+	888	WP_208795113.1	LysR substrate-binding domain-containing protein	-
J6U32_RS09905 (J6U32_09905)	2216731..2217729	+	999	WP_244332767.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-66)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10518.96 Da
• Isoelectric Point: 9.0879

>T197517 WP_208795107.1 NZ_CP072203:c2213754-2213473 [Gordonia polyisoprenivorans]
VIQSFATKDTERVWARKFVPRLGPELTRVAHRKLQALDAAQRLADLASPPGNRLEPLSGDRAGQHSIRVNDQYRLCFTWT
PVGPVDVEIVDYH

Nucleotide

• Length: 282 bp

>T197517 NZ_CP096231:c100649-100179 [Klebsiella pneumoniae subsp. pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
TTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10688.14 Da
• Isoelectric Point: 5.5882

>AT197517 WP_208795105.1 NZ_CP072203:c2213457-2213173 [Gordonia polyisoprenivorans]
MPDFEPIHPGETLAADYLPDYGLSQRKLAELIHVPPRRINEIIHGRRAITPDTSIRLGRLFGQSETFWLNMQTRYDLEIA
RATTDVSDITPLAS

Nucleotide

• Length: 285 bp

>AT197517 NZ_CP096231:c101089-100646 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAATCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACGTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACAGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA