Detailed information of TA system TA197494

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 358002..358648
• Replicon: chromosome
• Accession: NZ_CP072200
• Organism: Klebsiella pneumoniae strain B5055

Toxin (Protein)

• Gene name: higB
• Uniprot ID: W9BBY1
• Locus tag: J6T75_RS01655
• Protein ID: WP_016529833.1
• Coordinates: 358002..358349 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: W9BB18
• Locus tag: J6T75_RS01660
• Protein ID: WP_016529832.1
• Coordinates: 358349..358648 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6T75_RS01645 (J6T75_01635)	353928..355361	+	1434	WP_002920564.1	glycogen synthase GlgA	-
J6T75_RS01650 (J6T75_01640)	355379..357826	+	2448	WP_002920561.1	glycogen phosphorylase	-
J6T75_RS01655 (J6T75_01645)	358002..358349	+	348	WP_016529833.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J6T75_RS01660 (J6T75_01650)	358349..358648	+	300	WP_016529832.1	helix-turn-helix transcriptional regulator	Antitoxin
J6T75_RS01665 (J6T75_01655)	358711..360219	-	1509	WP_002920554.1	glycerol-3-phosphate dehydrogenase	-
J6T75_RS01670 (J6T75_01660)	360424..360753	+	330	WP_002920552.1	thiosulfate sulfurtransferase GlpE	-
J6T75_RS01675 (J6T75_01665)	360804..361634	+	831	WP_004151408.1	rhomboid family intramembrane serine protease GlpG	-
J6T75_RS01680 (J6T75_01670)	361684..362442	+	759	WP_016529569.1	DeoR/GlpR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-84)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13549.56 Da
• Isoelectric Point: 5.6749

>T197494 WP_016529833.1 NZ_CP072200:358002-358349 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGDKDGMNEKRFYKEMITLADREFSQHLTKER

Nucleotide

• Length: 348 bp

>T197494 NZ_CP096219:214892-215158 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAAACGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTAGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10800.63 Da
• Isoelectric Point: 10.4041

>AT197494 WP_016529832.1 NZ_CP072200:358349-358648 [Klebsiella pneumoniae]
MATLKELMAKQSPESQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQTDNAPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Nucleotide

• Length: 300 bp

>AT197494 NZ_CP096219:214629-214892 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTGGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTAGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Structures

Toxin

• AlphaFold DB: W9BBY1

Antitoxin

• AlphaFold DB: W9BB18