Detailed information of TA system TA197477

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1072363..1073093
• Replicon: chromosome
• Accession: NZ_CP072199
• Organism: Serratia marcescens subsp. marcescens ATCC 13880

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A8B4GR63
• Locus tag: J6N75_RS05175
• Protein ID: WP_004937033.1
• Coordinates: 1072363..1072674 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J6N75_RS05180
• Protein ID: WP_015379178.1
• Coordinates: 1072671..1073093 (+)
• Length: 141 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6N75_RS05145 (J6N75_05145)	1067676..1068401	+	726	WP_004937040.1	LPS export ABC transporter ATP-binding protein	-
J6N75_RS05150 (J6N75_05150)	1068454..1069887	+	1434	WP_021505109.1	RNA polymerase factor sigma-54	-
J6N75_RS05155 (J6N75_05155)	1069911..1070198	+	288	WP_004937038.1	ribosome hibernation promoting factor	-
J6N75_RS05160 (J6N75_05160)	1070354..1070815	+	462	WP_016930212.1	PTS IIA-like nitrogen regulatory protein PtsN	-
J6N75_RS05165 (J6N75_05165)	1070945..1071799	+	855	WP_015379177.1	RNase adapter RapZ	-
J6N75_RS05170 (J6N75_05170)	1071796..1072068	+	273	WP_004937034.1	PTS phosphocarrier protein NPr	-
J6N75_RS05175 (J6N75_05175)	1072363..1072674	+	312	WP_004937033.1	type II toxin-antitoxin system HigB family toxin	Toxin
J6N75_RS05180 (J6N75_05180)	1072671..1073093	+	423	WP_015379178.1	helix-turn-helix domain-containing protein	Antitoxin
J6N75_RS05185 (J6N75_05185)	1073097..1075013	-	1917	WP_033639303.1	PRD domain-containing protein	-
J6N75_RS05190 (J6N75_05190)	1075065..1076279	-	1215	WP_033639302.1	lactonase family protein	-
J6N75_RS05195 (J6N75_05195)	1076556..1077296	-	741	WP_016930215.1	KDGP aldolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (81-135)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12289.14 Da
• Isoelectric Point: 10.0957

>T197477 WP_004937033.1 NZ_CP072199:1072363-1072674 [Serratia marcescens subsp. marcescens ATCC 13880]
MHIISREPFNNATKQYPNEATALEAIYKALRRGEFRTPDELKALFPSLDRMKYKEKWWVIDVGGNHLRILFFASFETQKV
FIKHIVSHAEYDKLMQHYRRSSS

Nucleotide

• Length: 312 bp

>T197477 NZ_CP096201:c4982293-4981922 [Kluyvera ascorbata]
ATGAAAAGACCCGTTCTTTTTGATACCAATATTTTAATCGATCTCTTCAGCGGGCGGATGGAGGCGAAGCGGGCGATGGA
TTCCTACCCTCCGCAACATGCGATAAGCCTGATCACCTGGATGGAGGTCATGATAGGCGCAAAGCGCTTTCATCAGGAGC
AGCGAACGCGAATTGCCTTGAGCGCATTCGAAATTATTGGTGTAACAGAAGAAATTGCTGAGCAGAGTGTGAATCTGCGG
CAGGAGTACGGCATGAAGTTGCCCGATGCCATTATTCTGGCCACGGCGCATGTTCATCGCCGGGAACTGGTGACGCGTAA
CACAAAGGATTTTGCGGGGCTTCCGGGCGTGATTTTACCCTACGCGCTGTAA

Antitoxin

Protein

• Length: 141 a.a.
• Molecular weight: 15784.13 Da
• Isoelectric Point: 5.2139

>AT197477 WP_015379178.1 NZ_CP072199:1072671-1073093 [Serratia marcescens subsp. marcescens ATCC 13880]
MITDAIKAADALLRAVPLLQGSRAQQDYREALELVEYLLENDEHHPLIDMLAKKIAEYEDNSAEFTAFNQRIAQLPQGVA
ALRVLMEQHQLSQSDFEHEIGKKSLVNQILNGKRSLTIPHIMALAKRFNLPPALFLPEAD

Nucleotide

• Length: 423 bp

>AT197477 NZ_CP096201:c4982538-4982290 [Kluyvera ascorbata]
ATGATGGTGGAAAGGGACATGGGCAGAATACTTCTCGATTTGTCGGACGAGGTGATTAAGCGCCTCGACATACTTAAACA
GCAGCGAAATCTGCCTCGTGCGGAACTGCTGCGTGAAGCCGTTGAGCAATATCTTGAAAAGCAGAGTCAGGCTGAGCTCA
CTATTTCTGGCGCGCTTGGCATCTGGCAAGGGTGTGAAGAAGATAGCGTTGAGTATGAAAGAAAACTTCGGGAAGAGTGG
CCGCGATGA

Structures

Toxin

• AlphaFold DB: A0A8B4GR63