Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-DinJ
Location 1315048..1315612 Replicon chromosome
Accession NZ_CP072181
Organism Lacticaseibacillus paracasei strain HL182

Toxin (Protein)


Gene name yafQ Uniprot ID A0A510WIJ9
Locus tag J3H89_RS06625 Protein ID WP_016363448.1
Coordinates 1315310..1315612 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID K6SLT0
Locus tag J3H89_RS06620 Protein ID WP_003570051.1
Coordinates 1315048..1315317 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J3H89_RS06590 (J3H89_001318) 1310053..1310940 - 888 WP_003570041.1 ABC transporter ATP-binding protein -
J3H89_RS06595 (J3H89_001319) 1311137..1312363 - 1227 WP_003570043.1 hypothetical protein -
J3H89_RS06600 (J3H89_001320) 1312561..1312965 + 405 WP_003570045.1 MerR family transcriptional regulator -
J3H89_RS06605 (J3H89_001321) 1312958..1313284 + 327 WP_003584272.1 YrdB family protein -
J3H89_RS06610 (J3H89_001322) 1313347..1313976 + 630 WP_003570047.1 DUF2399 domain-containing protein -
J3H89_RS06615 (J3H89_001323) 1314021..1314674 + 654 WP_003570049.1 N-acetyltransferase -
J3H89_RS06620 (J3H89_001324) 1315048..1315317 + 270 WP_003570051.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
J3H89_RS06625 (J3H89_001325) 1315310..1315612 + 303 WP_016363448.1 type II toxin-antitoxin system YafQ family toxin Toxin
J3H89_RS06630 (J3H89_001326) 1315951..1316094 + 144 WP_162259784.1 hypothetical protein -
J3H89_RS06635 (J3H89_001327) 1316212..1316904 + 693 WP_003570055.1 hypothetical protein -
J3H89_RS06640 (J3H89_001328) 1317140..1319317 + 2178 WP_003570057.1 Tex family protein -
J3H89_RS06645 (J3H89_001329) 1319742..1320299 - 558 WP_003584278.1 helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(3-97)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(10-70)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11510.33 Da        Isoelectric Point: 9.8881

>T197471 WP_016363448.1 NZ_CP072181:1315310-1315612 [Lacticaseibacillus paracasei]
MYSLVPTPTFKRDLKRLSKKHWPMDELKTAVNLLAAGTNAELLSKKYADHALSSSSEWKGYRELYVDGPRGDWLLIYKIE
QQDLILTLVRTGSHHNLLVK

Download         Length: 303 bp

>T197471 NZ_CP096201:c3162093-3161797 [Kluyvera ascorbata]
ATGCTTGTAAACATTGGAAGCTTTCGAGATGACTGGCTTGCGGATTTTTTTAAACATGCAACACCACATAAAAAAATCCC
CTCAGACATACATGCATCTCTGGCCAGGAAGCTAGATATCATTAATGCAGCGGTAACTTACCAAGACTTGAAGTCACCGC
CCGGTAACCGTTATGAGGAACTTGAGGGAAAACTCAAAGATTATTCTTCAATAAGGGTTAACAAGCAGTACCGATTAATT
TTCCAGTGGGCAAATGGTAAAGCGCACGATCTTTATTTAGATCCACATGAATACTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 9982.68 Da        Isoelectric Point: 7.2026

>AT197471 WP_003570051.1 NZ_CP072181:1315048-1315317 [Lacticaseibacillus paracasei]
MAATKKETRLNIRVDPELKSAAQIVANDMGIDLTAAVTMFMTKMVKDHALPFTPTSLPVETLQALKEAKHPELLKKYSTP
DDMWRDLNV

Download         Length: 270 bp

>AT197471 NZ_CP096201:c3161730-3161389 [Kluyvera ascorbata]
ATGAATCAAGCAACCAGAAAGCCGACTACGGTAGGGGACATCCTTCTGTTTGAATACCTGGAGCCGCTTGACCTTAAAAT
CAACGATCTGGCGGAAATGCTGCAGGTACACCGCAACACGGTCAGCGCGCTAGTGAACAATAATCGTAAGCTGACTATTG
ATATGGCGTTTCGCCTGGCTAAAGCGTTTGATACCTCGGCTGCATTTTGGATTAATTTACAGGCAGCTGTCGATGTATGG
GAAACCGAAAACGACGCTCGTCTTCAGGTTGAACTCAATAAAATTATTACGGCAGAAGATTTCCTGGCCGCACGCGCGAA
AGCGAATGAGAAAGCAGCGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A510WIJ9

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2BRK6

References