Detailed information of TA system TA197414

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2078490..2079081
• Replicon: chromosome
• Accession: NZ_CP072151
• Organism: Rhizobium sp. K15/93

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: J5280_RS10165
• Protein ID: WP_209182967.1
• Coordinates: 2078797..2079081 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: J5280_RS10160
• Protein ID: WP_209182968.1
• Coordinates: 2078490..2078786 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5280_RS10145 (J5280_10140)	2073534..2075351	+	1818	WP_209182970.1	methyl-accepting chemotaxis protein	-
J5280_RS10150 (J5280_10145)	2075521..2076117	+	597	WP_142824153.1	histidine phosphatase family protein	-
J5280_RS10155 (J5280_10150)	2076150..2078384	-	2235	WP_209182969.1	glycine--tRNA ligase subunit beta	-
J5280_RS10160 (J5280_10155)	2078490..2078786	-	297	WP_209182968.1	HigA family addiction module antitoxin	Antitoxin
J5280_RS10165 (J5280_10160)	2078797..2079081	-	285	WP_209182967.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J5280_RS10170 (J5280_10165)	2079163..2081106	-	1944	WP_209183018.1	DUF2207 domain-containing protein	-
J5280_RS10175 (J5280_10170)	2081152..2082426	-	1275	WP_209182966.1	3-phosphoshikimate 1-carboxyvinyltransferase	-
J5280_RS10180 (J5280_10175)	2082522..2083073	-	552	WP_209182965.1	LemA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (15-94)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-92)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10505.98 Da
• Isoelectric Point: 7.2774

>T197414 WP_209182967.1 NZ_CP072151:c2079081-2078797 [Rhizobium sp. K15/93]
MVILSFKDGSTRAVASGVVPKGFPADLARRAIRKLTMIEYANQLSDLRSPPANHLEALKGDRAGQHSIRINDQWRICFVW
TDAGVEDVEIVDYH

Nucleotide

• Length: 285 bp

>T197414 NZ_CP096163:2399894-2400334 [Shewanella sp. JNE10-2]
GTGGAAGAGCAGCACCATAGTTTCAGCGTAAAAGCCTCCTTCGACCAACGGCTCTCGTTGGTCGTTTTTATGTGCGTTTG
TTCCTCATCATTCCTTATTTGGCCTTATACAGAACATTGGTTAGTGACGCTGCTACGGCTTGGATTGTTTTTGTTATCCG
TTAGCTTTTTGATATGGCAATTGTGGAGGCTTAAGGACTGGCGTTTAAGCTTTATGCTTAATGGTAAAGGTGACGGTCGA
TTATCCACGGGAGAACATTTCAGAATTTTAAGGCGCACATGGGTTACCCCATTTGTATGTATGATATACATCGATGTTGA
AGAAAATACGCGTTTAGTATTGCTTTGGGCCGATATGTTTACGGGTGTTGACTATAGACATTTATGCCGATTATTGCTTA
AGGCTAAAATGCTGCAAGCTAAATCTCAGGTTGAAATTTAG

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11204.13 Da
• Isoelectric Point: 8.6091

>AT197414 WP_209182968.1 NZ_CP072151:c2078786-2078490 [Rhizobium sp. K15/93]
MKHILPPVHPGEILREEYLAPLKMSAGALARKLNVPRTRIERLVAEETPVTIDTALRLARFFDTTPEFWMNMQTGYELKR
QAEEKRAEIAAIPVLHVA

Nucleotide

• Length: 297 bp

>AT197414 NZ_CP096163:2399665-2399913 [Shewanella sp. JNE10-2]
TTGGAATTAATGAATATTGCACGGGTTCGTTGGGCATGTCGTCGTGGAATGTTGGAATTGGATGTGCTGTTTCAACCTTT
CGTCGAGAATATTTATCAAGATTTGTCAGATATAGATAAAGCATCGTTTATTCGATTATTAGAATGTGAAGATCCTGAGT
TGTTTGCTTGGTTTATGGGGCATGAAGCCTGTCCTGATGCAGAGCTAGCTCGCATGGTTGTTAAAGTTCGTGGAAGAGCA
GCACCATAG