Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-VapI
Location 93178..93798 Replicon plasmid pTiL51_94
Accession NZ_CP072145
Organism Rhizobium sp. L51/94

Toxin (Protein)


Gene name graT Uniprot ID -
Locus tag J5274_RS27785 Protein ID WP_219276297.1
Coordinates 93178..93462 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag J5274_RS27790 Protein ID WP_219276299.1
Coordinates 93478..93798 (+) Length 107 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J5274_RS27755 (J5274_27760) 88284..88541 + 258 WP_219276289.1 type II toxin-antitoxin system VapB family antitoxin -
J5274_RS27760 (J5274_27765) 88538..88942 + 405 WP_219276291.1 type II toxin-antitoxin system VapC family toxin -
J5274_RS27765 (J5274_27770) 88939..89361 + 423 Protein_84 integrase -
J5274_RS27770 (J5274_27775) 89664..90773 - 1110 WP_219276293.1 IS110 family transposase -
J5274_RS27775 (J5274_27780) 90952..92075 + 1124 Protein_86 IS5 family transposase -
J5274_RS27780 (J5274_27785) 92258..92857 - 600 Protein_87 ornithine carbamoyltransferase -
J5274_RS29205 (J5274_27790) 92853..92942 - 90 Protein_88 recombinase family protein -
J5274_RS27785 (J5274_27795) 93178..93462 + 285 WP_219276297.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
J5274_RS27790 (J5274_27800) 93478..93798 + 321 WP_219276299.1 HigA family addiction module antitoxin Antitoxin
J5274_RS27795 (J5274_27805) 93832..94302 + 471 WP_219276301.1 site-specific DNA-methyltransferase -
J5274_RS27800 (J5274_27810) 94266..94691 - 426 Protein_92 transposase -
J5274_RS27805 (J5274_27815) 94820..95770 + 951 WP_219276302.1 IS630 family transposase -
J5274_RS27810 (J5274_27820) 96028..96594 - 567 WP_219276304.1 recombinase family protein -
J5274_RS27815 (J5274_27825) 96827..98446 - 1620 WP_219276306.1 hypothetical protein -
J5274_RS27820 (J5274_27830) 98502..98750 - 249 WP_219276308.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..279225 279225
- inside IScluster/Tn - - 90952..94595 3643


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(31-94)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(3-80)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10793.22 Da        Isoelectric Point: 6.9782

>T197398 WP_219276297.1 NZ_CP072145:93178-93462 [Rhizobium sp. L51/94]
MILSYKDKRTETFAAGGFVREFQGFNRQGWKRLEILDAATSLAELGQLPSNRLEALKADRAGQYSIRINMQWRICFEWPQ
DAAGPSNVEIVDYH

Download         Length: 285 bp

>T197398 NZ_CP096138:3069986-3070138 [Xanthomonas prunicola]
ATGAAGCGACTGCTGACACTGATGGTACTGGGCCTGTTTTCGGCCGGCGTGATGACGGGCTGCAACACCGTGGCTGGCGC
TGGCAAGGACATGCAGGGCGCAGGTGGGAAGGTCGAGAAAACCGCCGAAAAATGCAGCGACGGTAAGTGCTGA

Antitoxin


Download         Length: 107 a.a.        Molecular weight: 11704.23 Da        Isoelectric Point: 6.8790

>AT197398 WP_219276299.1 NZ_CP072145:93478-93798 [Rhizobium sp. L51/94]
MTRPAIHPGEILADELHELGITPTELSRQINVPPNRVTQIIKGQRNVTGDTALRLGHWFGMSAQFWLNLQSAYDIRLAEQ
TAGNEIAALPTRLANGAEHSRQPNSI

Download         Length: 321 bp

>AT197398 NZ_CP096138:3070213-3070347 [Xanthomonas prunicola]
ATGAAGCGTGCAATTGTGCTGCTGGTGCTGTCGATGTTGTCGGTCGGTATGCTGTCGGGCTGCAACACCGTCGCAGGTGC
TGGCAAGGACGTGAAGGGTGCTGGCGAGAAGGTGGAAGACGCAGCGCGTAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References