Detailed information of TA system TA197376

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 4084249..4084846
• Replicon: chromosome
• Accession: NZ_CP072130
• Organism: Klebsiella variicola strain F2R9

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0B7GEF2
• Locus tag: J5475_RS19800
• Protein ID: WP_012542526.1
• Coordinates: 4084529..4084846 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J5475_RS19795
• Protein ID: WP_012542525.1
• Coordinates: 4084249..4084536 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5475_RS19765 (J5475_19760)	4079887..4080135	+	249	WP_008805539.1	DUF1158 domain-containing protein	-
J5475_RS19770 (J5475_19765)	4080152..4080493	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
J5475_RS19775 (J5475_19770)	4080524..4081639	-	1116	WP_162499993.1	MBL fold metallo-hydrolase	-
J5475_RS19780 (J5475_19775)	4081819..4082400	+	582	WP_012968754.1	TetR/AcrR family transcriptional regulator	-
J5475_RS19785 (J5475_19780)	4082400..4082768	+	369	WP_008805536.1	MmcQ/YjbR family DNA-binding protein	-
J5475_RS19790 (J5475_19785)	4082888..4083541	+	654	WP_032729541.1	oxygen-insensitive NAD(P)H nitroreductase	-
J5475_RS19795 (J5475_19790)	4084249..4084536	-	288	WP_012542525.1	helix-turn-helix transcriptional regulator	Antitoxin
J5475_RS19800 (J5475_19795)	4084529..4084846	-	318	WP_012542526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J5475_RS19805 (J5475_19800)	4085031..4086074	-	1044	WP_016160437.1	DUF2157 domain-containing protein	-
J5475_RS19810 (J5475_19805)	4086604..4087470	-	867	WP_008805530.1	helix-turn-helix transcriptional regulator	-
J5475_RS19815 (J5475_19810)	4087579..4089006	+	1428	WP_040973773.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-89)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12110.39 Da
• Isoelectric Point: 11.2767

>T197376 WP_012542526.1 NZ_CP072130:c4084846-4084529 [Klebsiella variicola]
MFRLVVHVDVKKELQALPPIVQAKMIRQIDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T197376 NZ_CP096118:3548851-3549069 [Salmonella enterica subsp. enterica serovar Indiana]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCTGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10815.51 Da
• Isoelectric Point: 10.2212

>AT197376 WP_012542525.1 NZ_CP072130:c4084536-4084249 [Klebsiella variicola]
MSKKIIDWDTLKTELLSQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQILLY

Nucleotide

• Length: 288 bp

>AT197376 NZ_CP096118:3548449-3548823 [Salmonella enterica subsp. enterica serovar Indiana]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCCAATCCTGTTAGTTTGTCTTGTTAA

Structures

Toxin

• AlphaFold DB: A0A0B7GEF2