Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 354687..355333 Replicon chromosome
Accession NZ_CP072130
Organism Klebsiella variicola strain F2R9

Toxin (Protein)


Gene name higB Uniprot ID A0A1F2M0N0
Locus tag J5475_RS01625 Protein ID WP_008806991.1
Coordinates 354687..355034 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1F2LZT5
Locus tag J5475_RS01630 Protein ID WP_008806992.1
Coordinates 355034..355333 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J5475_RS01615 (J5475_01615) 350575..352008 + 1434 WP_004202133.1 glycogen synthase GlgA -
J5475_RS01620 (J5475_01620) 352026..354473 + 2448 WP_008806990.1 glycogen phosphorylase -
J5475_RS01625 (J5475_01625) 354687..355034 + 348 WP_008806991.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
J5475_RS01630 (J5475_01630) 355034..355333 + 300 WP_008806992.1 XRE family transcriptional regulator Antitoxin
J5475_RS01635 (J5475_01635) 355396..356904 - 1509 WP_032740278.1 glycerol-3-phosphate dehydrogenase -
J5475_RS01640 (J5475_01640) 357109..357438 + 330 WP_004202138.1 thiosulfate sulfurtransferase GlpE -
J5475_RS01645 (J5475_01645) 357489..358319 + 831 WP_008806994.1 rhomboid family intramembrane serine protease GlpG -
J5475_RS01650 (J5475_01650) 358369..359127 + 759 WP_008806995.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13504.52 Da        Isoelectric Point: 6.2327

>T197363 WP_008806991.1 NZ_CP072130:354687-355034 [Klebsiella variicola]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFGPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T197363 NZ_CP096049:53964-54065 [Enterococcus faecalis]
GTGAAAGATTTAATGTCGTTGGTTATCGCACCGATCTTTGTAGGATTGGTTCTGGAAATGATTTCTCGTGTGTTGGACGA
GGAAGACGATAGCCGAAAGTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10814.61 Da        Isoelectric Point: 10.1464

>AT197363 WP_008806992.1 NZ_CP072130:355034-355333 [Klebsiella variicola]
MATLKELMAKQSPESQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT197363 NZ_CP096049:c54169-54105 [Enterococcus faecalis]
AACGACATTAAATCGTACAAATAACACAAAAAGCAATCCTACGGCGAATAGGATTGCTTTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2M0N0

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2LZT5

References