Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 40719..41369 Replicon chromosome
Accession NZ_CP072119
Organism Klebsiella michiganensis strain 12084

Toxin (Protein)


Gene name higB Uniprot ID A0A0H3H3K0
Locus tag J5O09_RS00195 Protein ID WP_014227314.1
Coordinates 40719..41060 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H3H3Q5
Locus tag J5O09_RS00200 Protein ID WP_014227313.1
Coordinates 41070..41369 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J5O09_RS00180 (J5O09_00180) 36711..38030 + 1320 WP_014227316.1 MFS transporter -
J5O09_RS00185 (J5O09_00185) 38176..39567 + 1392 WP_004126843.1 hexose-6-phosphate:phosphate antiporter -
J5O09_RS00190 (J5O09_00190) 40016..40468 + 453 WP_014839842.1 DUF1198 domain-containing protein -
J5O09_RS00195 (J5O09_00195) 40719..41060 + 342 WP_014227314.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
J5O09_RS00200 (J5O09_00200) 41070..41369 + 300 WP_014227313.1 helix-turn-helix transcriptional regulator Antitoxin
J5O09_RS00205 (J5O09_00205) 41488..42669 + 1182 WP_014227312.1 purine ribonucleoside efflux pump NepI -
J5O09_RS00210 (J5O09_00210) 42702..43511 - 810 WP_004855618.1 phosphonoacetaldehyde hydrolase -
J5O09_RS00215 (J5O09_00215) 43521..44624 - 1104 WP_014227310.1 2-aminoethylphosphonate--pyruvate transaminase -
J5O09_RS00220 (J5O09_00220) 44765..45484 + 720 WP_076752428.1 phosphonate utilization transcriptional regulator PhnR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13110.00 Da        Isoelectric Point: 5.7545

>T197335 WP_014227314.1 NZ_CP072119:40719-41060 [Klebsiella michiganensis]
MWDVETTEVFDKWFEAQTEALKEDMLAAMVILSEYGPRLGRPFADTVNDSAFSNMKELRIQHQGRPIRAFFVFDPSRCGI
VLCAGDKTGLNEKRFYKDMIKLADAEYRKHLNQ

Download         Length: 342 bp

>T197335 NZ_CP096017:c2925242-2924892 [Alkalimarinus coralli]
TTGGTAAGGCGTAAGCAGTTTGATCGAGGCGATATCGTGAAAGTCTCACTCAACCCCGTGGTAGGCCGTGAAATGCAAGG
CAATTTCAGGCCCTGTATGGTGTTGTCTCCAGCAGCGTTTAATCGCTTGGGTACGGCGTTGGTTGCCCCGATTACGCAAG
GCGGTGATTTCGCCAGAGTGAAGGGCTTTGCAGTTCCGCTAATTGGCTCCGGTACAGAGACTCAAGGCGTTATGCTGGTG
AATGCAGTACGAATGCTGGATCTGGAAGGCCGGCAAGCAAAAAAGGTTGAGGTCGCCCCCCAAGAGATCGTGGACGATGT
ATTGGCCCGACTTGCGGCCATCATCGAATAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10966.87 Da        Isoelectric Point: 9.7256

>AT197335 WP_014227313.1 NZ_CP072119:41070-41369 [Klebsiella michiganensis]
MATLKELMAKQSPESQARIAEKVEEMRQAIALSMLREELNMSQAELAKAMGVKQPTIAKMEQADNDPRLSTLKRYVAALG
GELSIDVKLPTGKRIAFHL

Download         Length: 300 bp

>AT197335 NZ_CP096017:c2925493-2925236 [Alkalimarinus coralli]
ATGAGAACTACAATTCGAAAGATTGGGAATAGCGCAGGTGCCATACTGCCAGCGACAGTCCTTAAGAAATTGAACTTGTC
AGAAGGCGATGTAATTGAGGTGTCGGAAGACGGCAAGCGTATTGTGATCGAGCCTCGCAATGAAAAGCCTAAATACAGCC
TGACAGAGCTTATGGCCCAGTGCGATGAAAAAGCCCCTCTACCCAAAGAACTGGAGGAATGGAATGACATGCAACCGACG
GGGAAAGAGGTTTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H3K0

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H3Q5

References