197244

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 252004..252698 Replicon chromosome
Accession NZ_CP072054
Organism Escherichia coli strain K1508

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag J5N95_RS01180 Protein ID WP_001263489.1
Coordinates 252300..252698 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag J5N95_RS01175 Protein ID WP_000554758.1
Coordinates 252004..252297 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J5N95_RS01155 (247636) 247636..248133 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
J5N95_RS01160 (248357) 248357..250069 - 1713 Protein_226 flagellar biosynthesis protein FlhA -
J5N95_RS01165 (250041) 250041..250826 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
J5N95_RS01170 (250897) 250897..251952 + 1056 WP_001226164.1 DNA polymerase IV -
J5N95_RS01175 (252004) 252004..252297 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
J5N95_RS01180 (252300) 252300..252698 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
J5N95_RS01185 (252708) 252708..253160 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
J5N95_RS01190 (253478) 253478..253684 + 207 Protein_232 RtcB family protein -
J5N95_RS01195 (253680) 253680..254201 + 522 Protein_233 peptide chain release factor H -
J5N95_RS01200 (254258) 254258..255715 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
J5N95_RS01205 (255976) 255976..256434 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- (257030) 257030..257110 + 81 NuclAT_12 - -
- (257030) 257030..257110 + 81 NuclAT_12 - -
- (257030) 257030..257110 + 81 NuclAT_12 - -
- (257030) 257030..257110 + 81 NuclAT_12 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T197244 WP_001263489.1 NZ_CP072054:252300-252698 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T197244 NZ_CP095843:c2687730-2687578 [Escherichia fergusonii]
ATGCCGCAGAAATACGGATTACTTTCGTTAATAGTGATTTGTTTCACGCTTTTATTTTTCACCTGGATGATAAGAGATTC
ACTGTGTGAATTGCATATTAAACAGGAGAGTTATGAGCTGGCGGCGTTTTTAGCCTGCAAATTGAAAGAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT197244 WP_000554758.1 NZ_CP072054:252004-252297 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT197244 NZ_CP095843:2687781-2687836 [Escherichia fergusonii]
GTTGAAGCATAGAGGCAGCCTCACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References