Detailed information of TA system TA197239

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 5719692..5720361
• Replicon: chromosome
• Accession: NZ_CP072011
• Organism: Pseudomonas corrugata strain 3prime

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: C4C32_RS25335
• Protein ID: WP_208555010.1
• Coordinates: 5719996..5720361 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A3M3EAW2
• Locus tag: C4C32_RS25330
• Protein ID: WP_024779393.1
• Coordinates: 5719692..5720003 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4C32_RS25320 (C4C32_25320)	5715736..5719479	+	3744	WP_055136376.1	EAL domain-containing protein	-
C4C32_RS25330 (C4C32_25330)	5719692..5720003	-	312	WP_024779393.1	XRE family transcriptional regulator	Antitoxin
C4C32_RS25335 (C4C32_25335)	5719996..5720361	-	366	WP_208555010.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4C32_RS25340 (C4C32_25340)	5720645..5723008	+	2364	WP_244251308.1	hypothetical protein	-
C4C32_RS25345 (C4C32_25345)	5723045..5723506	+	462	WP_024779396.1	Lrp/AsnC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (5-86)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 14019.69 Da
• Isoelectric Point: 5.5374

>T197239 WP_208555010.1 NZ_CP072011:c5720361-5719996 [Pseudomonas corrugata]
MKWDVEYTDEFEIWWNRLGESEQDSVQASVMLLGDAGPFLGFPHTSDIKGSRHGNLRELRVQHGGRPYRVLYAFDPHRCA
ILLIGGGKTGQDRWYHEYVPLAERLYDEHLEVLKKEGFDNG

Nucleotide

• Length: 366 bp

>T197239 NZ_CP095843:c1897349-1896942 [Escherichia fergusonii]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGTTAAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCGCCGTGGATGATTAAAAGCGGCATGATGTTGCGTTTACGTTCTGATAGCGGTAAACGTCA
GCACCTGTGGCTGGCAGCCGACAGCATGGACGAAGCAGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11825.59 Da
• Isoelectric Point: 9.9873

>AT197239 WP_024779393.1 NZ_CP072011:c5720003-5719692 [Pseudomonas corrugata]
MAKKFADLVARRSPDSQVRTQALYQKLRAEMPLHELRQAQELSQEALAKALHINQAAVSKMERRTDMYISTLRNYIRAMG
GELEIIATFPDGQVRIENFSHQP

Nucleotide

• Length: 312 bp

>AT197239 NZ_CP095843:c1897596-1897330 [Escherichia fergusonii]
ATGGACATTAATAACAAAGCCCGCATTCACTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGTGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTCAACTGGCTGATGAATCATGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A3M3EAW2