Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tsbAT/-
Location 970171..970973 Replicon chromosome
Accession NZ_CP071994
Organism Staphylococcus epidermidis strain AZ22

Toxin (Protein)


Gene name tsbT Uniprot ID Q5HN83
Locus tag J4Z28_RS04680 Protein ID WP_002468490.1
Coordinates 970794..970973 (+) Length 60 a.a.

Antitoxin (Protein)


Gene name tsbA Uniprot ID A0A8B5RW65
Locus tag J4Z28_RS04675 Protein ID WP_002470400.1
Coordinates 970171..970770 (+) Length 200 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J4Z28_RS04650 (J4Z28_000931) 965382..966839 + 1458 WP_002470402.1 ABC transporter substrate-binding protein/permease -
J4Z28_RS04655 (J4Z28_000932) 966832..967554 + 723 WP_001829838.1 amino acid ABC transporter ATP-binding protein -
J4Z28_RS04660 (J4Z28_000933) 968069..968206 + 138 WP_064783702.1 hypothetical protein -
J4Z28_RS04665 (J4Z28_000934) 968416..969546 + 1131 WP_002470398.1 tRNA epoxyqueuosine(34) reductase QueG -
J4Z28_RS04670 (J4Z28_000935) 969543..970013 + 471 WP_001829836.1 tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL -
J4Z28_RS04675 (J4Z28_000936) 970171..970770 + 600 WP_002470400.1 hypothetical protein Antitoxin
J4Z28_RS04680 (J4Z28_000937) 970794..970973 + 180 WP_002468490.1 SAS053 family protein Toxin
J4Z28_RS04685 (J4Z28_000938) 971129..971533 + 405 WP_001829818.1 hypothetical protein -
J4Z28_RS04690 (J4Z28_000939) 971718..973103 + 1386 WP_001829862.1 class II fumarate hydratase -
J4Z28_RS04695 (J4Z28_000940) 973464..974288 - 825 WP_001829804.1 RluA family pseudouridine synthase -
J4Z28_RS04700 (J4Z28_000941) 974447..975580 + 1134 WP_001829819.1 GAF domain-containing sensor histidine kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 968051..968206 155


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Glucosamine-6-phosphate isomerase

(130-195)


Sequences

Toxin        


Download         Length: 60 a.a.        Molecular weight: 6963.56 Da        Isoelectric Point: 4.3016

>T197234 WP_002468490.1 NZ_CP071994:970794-970973 [Staphylococcus epidermidis]
MANKKDSKLNYHEEENAMVTDLDDLKELGKEMEQISQENDEEKLNQSHDNEVRSDLKKQ

Download         Length: 180 bp

>T197234 NZ_CP095843:223038-223141 [Escherichia fergusonii]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 200 a.a.        Molecular weight: 22699.58 Da        Isoelectric Point: 4.9942

>AT197234 WP_002470400.1 NZ_CP071994:970171-970770 [Staphylococcus epidermidis]
MAMNFKVFDNSEKVAEYTADILRKQFNNNPTTIAGFHLSKEHAPVFDELKKNVENHTVDFSQINILDYDDNHSYYEALGV
PTGQIYSISYENDAIDFISDKIKTKENKGKLTMQVLTIDENGKLDISVRQGLMEAREIFLVITGANKRDMVEKLYRENGK
TSFEPSDLKAHRMVNVILDKEAAAGLSEDVKEYFTARFA

Download         Length: 600 bp

>AT197234 NZ_CP095843:c223179-222911 [Escherichia fergusonii]
AAAGTCAGCGAATGGAATATTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2G7HY44

Antitoxin

Source ID Structure
AlphaFold DB A0A8B5RW65

References