Detailed information of TA system TA197200

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 282704..283239
• Replicon: plasmid p1VB13828
• Accession: NZ_CP071959
• Organism: Enterococcus faecium strain VB13828

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: R2PQC0
• Locus tag: J4767_RS14835
• Protein ID: WP_002314366.1
• Coordinates: 282704..282976 (-)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: Q3Y220
• Locus tag: J4767_RS14840
• Protein ID: WP_002297422.1
• Coordinates: 282976..283239 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J4767_RS14795 (J4767_14795)	277811..278260	-	450	WP_010729386.1	TlpA disulfide reductase family protein	-
J4767_RS14800 (J4767_14800)	278416..278958	-	543	WP_002298088.1	hypothetical protein	-
J4767_RS14805 (J4767_14805)	279233..279876	+	644	Protein_324	transposase	-
J4767_RS14810 (J4767_14810)	279952..280632	+	681	WP_002331383.1	IS6-like element IS1216 family transposase	-
J4767_RS14815 (J4767_14815)	280691..280879	-	189	Protein_326	hypothetical protein	-
J4767_RS14820 (J4767_14820)	280880..281737	-	858	WP_002292680.1	ParA family protein	-
J4767_RS14825 (J4767_14825)	282022..282308	-	287	Protein_328	type II toxin-antitoxin system RelE/ParE family toxin	-
J4767_RS14830 (J4767_14830)	282298..282627	-	330	WP_002292678.1	AbrB family transcriptional regulator	-
J4767_RS14835 (J4767_14835)	282704..282976	-	273	WP_002314366.1	Txe/YoeB family addiction module toxin	Toxin
J4767_RS14840 (J4767_14840)	282976..283239	-	264	WP_002297422.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
J4767_RS14845 (J4767_14845)	283473..284381	+	909	Protein_332	IS982-like element ISEfm1 family transposase	-
J4767_RS14850 (J4767_14850)	284384..285559	-	1176	WP_002336205.1	MFS transporter	-
J4767_RS14855 (J4767_14855)	285562..287811	-	2250	WP_002314387.1	alpha-galactosidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	tet(L) / ant(6)-Ia / aph(3')-III / erm(B) / aac(6')-aph(2'')	-	1..296092	296092
	-	inside	IScluster/Tn	-	-	271142..293840	22698

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (9-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (5-69)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10780.24 Da
• Isoelectric Point: 9.9724

>T197200 WP_002314366.1 NZ_CP071959:c282976-282704 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Nucleotide

• Length: 273 bp

>T197200 NZ_CP095776:c1064137-1063919 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9959.22 Da
• Isoelectric Point: 4.5370

>AT197200 WP_002297422.1 NZ_CP071959:c283239-282976 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Nucleotide

• Length: 264 bp

>AT197200 NZ_CP095776:c1064538-1064164 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A829F704

Antitoxin

• AlphaFold DB: A0A829F006