Detailed information of TA system TA197189

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 245120..245710
• Replicon: chromosome
• Accession: NZ_CP071957
• Organism: Alcaligenes faecalis strain JLAF9

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0A2N9E1
• Locus tag: J5J84_RS01105
• Protein ID: WP_035268575.1
• Coordinates: 245414..245710 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: J5J84_RS01100
• Protein ID: WP_252606603.1
• Coordinates: 245120..245407 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5J84_RS01085 (J5J84_01090)	240910..242157	+	1248	WP_045930567.1	D-amino acid dehydrogenase	-
J5J84_RS01090 (J5J84_01095)	242353..243363	-	1011	WP_252605852.1	acyltransferase family protein	-
J5J84_RS01095 (J5J84_01100)	243642..245009	+	1368	WP_252605853.1	DNA recombination protein RmuC	-
J5J84_RS01100 (J5J84_01105)	245120..245407	-	288	WP_252606603.1	HigA family addiction module antitoxin	Antitoxin
J5J84_RS01105 (J5J84_01110)	245414..245710	-	297	WP_035268575.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J5J84_RS01110 (J5J84_01115)	245844..248402	-	2559	WP_252605854.1	EAL domain-containing protein	-
J5J84_RS01115 (J5J84_01120)	248586..248864	+	279	WP_042484640.1	acylphosphatase	-
J5J84_RS01120 (J5J84_01125)	249021..249917	+	897	WP_042484636.1	MurR/RpiR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-98)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (2-85)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11108.48 Da
• Isoelectric Point: 9.3459

>T197189 WP_035268575.1 NZ_CP071957:c245710-245414 [Alcaligenes faecalis]
MIKSFRHKGLQAYFETGSKSGIQAKHAVRLQIQLTALHSATQAEDMNAPGWKLHKLSGKNPKGQSVQDHYTISVSGNWRL
TFYFEGEDAVLVDYQDYH

Nucleotide

• Length: 297 bp

>T197189 NZ_CP095774:774121-774399 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10447.00 Da
• Isoelectric Point: 9.8929

>AT197189 WP_252606603.1 NZ_CP071957:c245407-245120 [Alcaligenes faecalis]
IMRMHNPSHPGLVLREYLGELSVTEAASRLGVTRAALSRILNGSAGISADMALRLRDALGTSAEMWINMQSHYDLWQAEQ
KPRPKVVSFHGPAHA

Nucleotide

• Length: 288 bp

>AT197189 NZ_CP095774:774411-774716 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAATGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: A0A0A2N9E1