Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 5024253..5024855 Replicon chromosome
Accession NZ_CP071954
Organism Escherichia coli strain SB0258h1

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag J5X85_RS24330 Protein ID WP_000897305.1
Coordinates 5024544..5024855 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag J5X85_RS24325 Protein ID WP_000356395.1
Coordinates 5024253..5024543 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J5X85_RS24290 (5019877) 5019877..5020779 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
J5X85_RS24295 (5020776) 5020776..5021411 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
J5X85_RS24300 (5021408) 5021408..5022337 + 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
J5X85_RS24305 (5022519) 5022519..5022761 - 243 WP_001309881.1 CopG family transcriptional regulator -
J5X85_RS24310 (5022980) 5022980..5023198 - 219 WP_001295676.1 CopG family transcriptional regulator -
J5X85_RS24315 (5023617) 5023617..5023895 - 279 WP_001315112.1 hypothetical protein -
J5X85_RS24320 (5023947) 5023947..5024168 - 222 WP_001550354.1 hypothetical protein -
J5X85_RS24325 (5024253) 5024253..5024543 - 291 WP_000356395.1 NadS family protein Antitoxin
J5X85_RS24330 (5024544) 5024544..5024855 - 312 WP_000897305.1 hypothetical protein Toxin
J5X85_RS24335 (5025084) 5025084..5025992 + 909 WP_001550353.1 alpha/beta hydrolase -
J5X85_RS25645 (5026160) 5026160..5027074 - 915 WP_109553727.1 transposase -
J5X85_RS25650 (5027087) 5027087..5027974 - 888 Protein_4813 hypothetical protein -
J5X85_RS24345 (5028390) 5028390..5029331 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
J5X85_RS24350 (5029376) 5029376..5029813 - 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T197181 WP_000897305.1 NZ_CP071954:c5024855-5024544 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

>T197181 NZ_CP095770:2170130-2170717 [Pseudomonas aeruginosa]
ATGTCGAGGAGGGCGCCCATGTTCAAGCCGCTACCGATCACCCTGCAGCGCGGAGCCTTGCGTCTCGAGCCGCTGGTGGA
AGCGGACATTCCCGAGCTGGTGAGCCTCGCCGAAGCCAACCGCGAGGCGCTGCAGTACATGGACGGACCGACGCGGCCGG
ACTGGTACCGCCAGAGCCTCGCCGAGCAGCGCGAGGGCAGGGCGTTGCCGCTCGCCGTCCGCCTCGGCGTGCAACTGGTC
GGGACCACGCGCTTCGCCGAATTCCTTCCCGCCTTGCCGGCCTGCGAGATCGGCTGGACCTGGCTCGACCAGGCCCAGCA
CGGCAGCGGCCTGAACCGCATGATCAAGTACCTGATGCTCAAGCATGCCTTCGACAACCTGCGCATGGTCCGCGTGCAGC
TCAGCACGGCGGCGAGCAACCTGCGCGCCCAGGGAGCCATCGACAAGCTCGGCGCGCAGCGCGAGGGCGTGCTGCGCAAC
CACCGGCGGCTGGCCGGCGGGCGGCTAGACGACACCTTCGTCTACAGCATCACCGACCACGAATGGCCGCAGGTCAAGGC
GGCGCTGGAAGCCAGCTTCACCGGCTGA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10869.46 Da        Isoelectric Point: 10.8907

>AT197181 WP_000356395.1 NZ_CP071954:c5024543-5024253 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp

>AT197181 NZ_CP095770:2170784-2171638 [Pseudomonas aeruginosa]
GTGCCGGACGAGACGAGCGGAGAACATACGCATTTCTGGCGCGCCGCCGAACTGGGCGGCGTCGAGTTGCTGCATGCGCG
CTACGTCGAGCAGCGTTTCGCCCCCCATGTGCACAGCGGCTTCGCCCTGGTCTTCATCGAGCAGGGCGCGCAGCGCTTCC
GCCATCGCGGCGGCGAGCACCTGGCGCCCTGGGGCAGCATGGTGCTGATCAACCCCGACGAAGTGCACACCGGCTCCAAG
GCCCATGACTCCGGCTGGCTCTATCGCGGCTACTACCCGGAGGTGGAGCAGATCGCCAGTATCCTCGACGAGCTGGAAAT
CCGCCACGGCGGGGTGCCGCGTTTCGCCGACAGCGTGGTCCATGACCAGCCCCTGGCGCGCGCCTTCGTCCATCTGCACC
GCTTGCTGGCCGACCACGCCGGCAGCCTGGAAACCCAGACCTGCTGGCGCGAGACGCTGCTCGCCCTGTTCCGTCGCCAC
GCCCGCCTGCCGGAGGCGCCGAAGGCCGGGTCGGAACCGTTGGCGGTCGCCCGCGCCCGCGAGTTGCTGGCGGCGCGTTT
GCTCGACCCGCCGTCGCTGGAAGAACTGGCGGCTGCGGTGAATCTGTCGCCGTTCCACTTCGCCCGGGTGTTCCGCCGCG
CCACCGGGATGCCGCCGCATGCCTGGCTCCGGCAACGGCGCCTGGAACAGGCCAGGGCGCTGCTCAAGAATGGCCGGGCG
CCGCTGGATGTCGCCCTGGAACTGGGCTTCGCCGACCAGAGCCATCTCGGTCGCCAGTTCAAGCAGGCCTACGGCGTGGC
GCCGGGCGAGTACCGCGCGGCCTGTGCGCGCTCGTCCTTGACGATGTGCGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References