Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4029415..4030109 Replicon chromosome
Accession NZ_CP071954
Organism Escherichia coli strain SB0258h1

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag J5X85_RS19560 Protein ID WP_001263491.1
Coordinates 4029415..4029813 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag J5X85_RS19565 Protein ID WP_000554755.1
Coordinates 4029816..4030109 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (4025244) 4025244..4025324 - 81 NuclAT_10 - -
- (4025244) 4025244..4025324 - 81 NuclAT_10 - -
- (4025244) 4025244..4025324 - 81 NuclAT_10 - -
- (4025244) 4025244..4025324 - 81 NuclAT_10 - -
J5X85_RS19530 (4024584) 4024584..4025828 - 1245 WP_000189541.1 esterase FrsA -
J5X85_RS19535 (4025920) 4025920..4026378 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
J5X85_RS19540 (4026639) 4026639..4028096 + 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
J5X85_RS19545 (4028153) 4028153..4028505 - 353 Protein_3875 peptide chain release factor H -
J5X85_RS19550 (4028501) 4028501..4028707 - 207 Protein_3876 RtcB family protein -
J5X85_RS19555 (4028953) 4028953..4029405 - 453 WP_019842500.1 GNAT family N-acetyltransferase -
J5X85_RS19560 (4029415) 4029415..4029813 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
J5X85_RS19565 (4029816) 4029816..4030109 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
J5X85_RS19570 (4030161) 4030161..4031216 - 1056 WP_001550655.1 DNA polymerase IV -
J5X85_RS19575 (4031287) 4031287..4032072 - 786 WP_019842510.1 putative lateral flagellar export/assembly protein LafU -
J5X85_RS19580 (4032044) 4032044..4033756 + 1713 Protein_3882 flagellar biosynthesis protein FlhA -
J5X85_RS19585 (4033861) 4033861..4034139 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
J5X85_RS19590 (4034132) 4034132..4034488 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T197176 WP_001263491.1 NZ_CP071954:c4029813-4029415 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T197176 NZ_CP095766:c4033626-4033057 [Pseudomonas chengduensis]
ATGTTCAAGCCGCAGCTGGTGACTTTGCAACGTGGCGCTCTGCGCCTGGAACCGCTCTCTGACGCGGATATCCCCGCGCT
GGTGGCGCTGGCCGAAGCCAACCGGGAGGAGCTGCTGTACATGAGCGGCACCCAGCGACTGGACTGGTACCGCAGCGCCC
TGGCCGATATGCGCGAGCAGCGGGCACTGCCGTTCGTCATTCGTCTGGGGGACGAGTTGGTAGGCACCACCCGCTTCGCC
GACTTCATGAACACCCTGCCGGCCTGTGAAATCGGCTGGACCTGGCTCGACCGCGGCCAGCATGGCAGCGGGTTGAACAC
CAGCATCAAGTACTTGATGCTCAAGCACGCTTTCGAAAATTGGGGCATGGTTCGCGTGCAACTGAAAACCGCCGCCAGCA
ACCTGCGTTCGCAACGCGCCATCGAGAAGCTTGGCGCCGTTCGTGAAGGGTTACTGCGCAACCATCGCCGTCTGGCTGAT
GGCCGACTCGATGACACCGTGCTGTACAGCATCACCGATCTGGAATGGCCGGCGCTGCGTGGGGCCATGGAGGCGGGCTT
CAACGCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT197176 WP_000554755.1 NZ_CP071954:c4030109-4029816 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT197176 NZ_CP095766:c4033051-4032224 [Pseudomonas chengduensis]
ATGCACAGTCGCGAGCAGACCCGTTTCTGGCAAGCTTCCGCATTGAGCGGCGTCGAGCTGTTGCAGGCGCGCTACCTTGA
ACAGCGCTTCGCGCCTCACGTGCATGAAGGTTTCGTGTTCACGGTGATTGAGCATGGCGCCCAGCGCTTTCGTCATCGCG
GTTGTGATCATCTGGCACCGCAGGGCAGCATGGTGCTGATCAACCCGGATGAAGTGCACACCGGCTCCAAGGCGCACGAA
GACGGATGGCGCTATCGCGGTTTCTATCCCGACAACGTCCAGGTACTGGGGGCGTTGCAGGAGTTGGGCCTGGCCAAGGC
TGGCATGCCGTCGTTTGCCTTCAGCGTGCTGCATGATCCACGTTTGCATGCGGCCTTTCTGCAACTGCACCAACTGCTCG
AGGGTGGTGCCGAGGCGCTGCAGCAGCAACTGGCCTGGCGTGAGGCGATTCTGCTGTTGTTTCAGCATCATGCGCAGCTT
GCCGAACCACCTGCGCCTGGGCGTGAGCCCTGGGCAGTGGCTTGTGCCAAGCAGATGCTCGCCGAGCGCCTGGTCGAGCC
GCCGTCTCTGGAAGAGCTGGCGGCTGCGGTTAGCCTCTCTCCTTTTCACTTCGCGCGCGTATTTCGCCGCGCGACCGGCT
TGCCGCCGCATGCCTGGCTCAAGCAGCGTCGCCTCGAACAGGCGCGGGCGTTGCTCAAGACGGGCTGTACGGCGGTCGCT
GTGGCCATGCAGTTGGGCTTCGCCGATCAGAGCCACTTGTCGCGGCAGTTCAAGCAGGTTTATGGCGTGAGCCCTGGCGA
ATATCGCAGCGCGGTGGCGCGGAGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References