Detailed information of TA system TA197159

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1171541..1172268
• Replicon: chromosome
• Accession: NZ_CP071954
• Organism: Escherichia coli strain SB0258h1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V0YLE2
• Locus tag: J5X85_RS05605
• Protein ID: WP_000547555.1
• Coordinates: 1171541..1171852 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J5X85_RS05610
• Protein ID: WP_000126294.1
• Coordinates: 1171849..1172268 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5X85_RS05580 (1167476)	1167476..1169185	+	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-
J5X85_RS05585 (1169195)	1169195..1169737	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
J5X85_RS05590 (1169737)	1169737..1170504	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
J5X85_RS05595 (1170501)	1170501..1170911	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
J5X85_RS05600 (1170904)	1170904..1171374	+	471	WP_001551542.1	hydrogenase maturation peptidase HycI	-
J5X85_RS05605 (1171541)	1171541..1171852	+	312	WP_000547555.1	type II toxin-antitoxin system HigB family toxin	Toxin
J5X85_RS05610 (1171849)	1171849..1172268	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
J5X85_RS05615 (1172347)	1172347..1173771	-	1425	WP_000110355.1	6-phospho-beta-glucosidase AscB	-
J5X85_RS05620 (1173780)	1173780..1175237	-	1458	WP_001107889.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
J5X85_RS05625 (1175497)	1175497..1176507	+	1011	WP_029130985.1	DNA-binding transcriptional regulator AscG	-
J5X85_RS05630 (1176656)	1176656..1177183	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12439.18 Da
• Isoelectric Point: 9.5146

>T197159 WP_000547555.1 NZ_CP071954:1171541-1171852 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T197159 NZ_CP095762:c4750805-4750521 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT197159 WP_000126294.1 NZ_CP071954:1171849-1172268 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT197159 NZ_CP095762:c4751037-4750795 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: V0YLE2