197146

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-VapI
Location 468766..469361 Replicon chromosome
Accession NZ_CP071947
Organism Pseudomonas aeruginosa strain 2020HL-00861

Toxin (Protein)


Gene name higB Uniprot ID A0A241XLJ5
Locus tag J5V74_RS02170 Protein ID WP_003117425.1
Coordinates 469083..469361 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag J5V74_RS02165 Protein ID WP_003113527.1
Coordinates 468766..469071 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J5V74_RS02140 (J5V74_02140) 464007..464273 + 267 WP_023088595.1 hypothetical protein -
J5V74_RS02145 (J5V74_02145) 464329..464664 + 336 WP_058153555.1 hypothetical protein -
J5V74_RS02150 (J5V74_02150) 465145..465960 - 816 WP_139268714.1 hypothetical protein -
J5V74_RS02155 (J5V74_02155) 466113..467408 - 1296 WP_180712070.1 hypothetical protein -
J5V74_RS02160 (J5V74_02160) 467515..468372 - 858 WP_073663727.1 restriction endonuclease -
J5V74_RS02165 (J5V74_02165) 468766..469071 - 306 WP_003113527.1 HigA family addiction module antitoxin Antitoxin
J5V74_RS02170 (J5V74_02170) 469083..469361 - 279 WP_003117425.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
J5V74_RS31355 469414..469542 - 129 Protein_421 integrase -
J5V74_RS02175 (J5V74_02175) 469690..471918 + 2229 WP_219879311.1 TonB-dependent receptor -
J5V74_RS02180 (J5V74_02180) 471988..472635 - 648 WP_003095021.1 carbonate dehydratase -
J5V74_RS02185 (J5V74_02185) 472697..473935 - 1239 WP_019681675.1 C69 family dipeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(5-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(22-69)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10701.28 Da        Isoelectric Point: 8.5576

>T197146 WP_003117425.1 NZ_CP071947:c469361-469083 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Download         Length: 279 bp

>T197146 NZ_CP095761:2200767-2200870 [Klebsiella michiganensis]
GGCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11247.81 Da        Isoelectric Point: 5.0747

>AT197146 WP_003113527.1 NZ_CP071947:c469071-468766 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Download         Length: 306 bp

>AT197146 NZ_CP095761:c2200877-2200709 [Klebsiella michiganensis]
AACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCCTTATAAGAATAGTTTACCGTGTCAGGTTTTCCAGTCCGCGACAAAAGTG
GACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A241XLJ5

Antitoxin

Source ID Structure

References